生黄合剂对缺血再灌注损伤大鼠心肌细胞凋亡相关基因Bax,Bcl-2和Caspase-3蛋白表达的影响  

Influence of Sheng-huang Mixtrue on Apoptosis Related Proteins Bax,Bcl-2 and Caspase-3 in Myocardial Ischemia-reperfusion Rats

作　　者：高玉峰[1] 王小杰[1] 闫文翠 张维娜[2] 李欣[1] 张凤英[1] 

机构地区：[1]承德医学院,河北承德067000 [2]承德护理职业学院,河北承德067000

出　　处：《中国实验方剂学杂志》2012年第13期244-247,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省科学技术研究与发展计划项目(10276141)

摘　　要：目的:观察生黄合剂对缺血再灌注损伤大鼠心肌细胞凋亡相关基因Bcl-2相关X蛋白(Bax)、B型白细胞/2型淋巴细胞样蛋白(Bcl-2)和天冬氨酸特异性半胱氨酸蛋白酶(Caspase-3)蛋白表达的影响。方法:将48只SD大鼠随机分为6组,即对照组、模型组、阳性药物组、生黄合剂低、中、高剂量组(17.5,35,70 mg·kg-1分别加入5 mL·kg-1生脉饮),每组8只,分别给药7 d后,建立心肌缺血再灌注损伤(MIRI)模型。采用原位末端标记法(TUNEL)检测心肌细胞凋亡情况,蛋白印迹法检测心肌细胞凋亡相关基因Bax,Bcl-2,Caspase-3蛋白的表达。结果:与模型组比较,生黄合剂能减少心肌细胞凋亡指数(AI)及Bax,Caspase-3蛋白的表达,增加Bcl-2蛋白的表达,提高Bcl-2/Bax的比值(P<0.05),其中以生黄合剂高剂量组作用较为明显。结论:生黄合剂对大鼠心肌缺血再灌注损伤具有保护作用,其抗心肌细胞凋亡的机制可能与通过上调Bcl-2,下调Bax和Caspase-3基因表达,提高Bcl-2/Bax的比值有关。proteins expression Objective： To investigate the influence of Sheng-huang mixtrue on of Bcl-assosiated x protein （Bax）, B-cell leukemia/lymphoma 2-like the apoptosis related proteins （Bcl-2） andAspartate-specific cysteinyl proteinase （Caspase-3） in myocardial ischemia-reperfusion injury rats. Method： SD rats （n =48） were divided randomly into six groups： normal control group, model group, positive control group, low-dose, medium-dose and high-dose Sheng-huang mixtrue groups （n = 8）. After the drug was administered to the six groups seven days, the Myocardial Isehemia-reperfusion injury in rats was established. TUNEL was used to detect the myocardial apoptosis index, The protein expressions of Bax, Bcl-2 and Caspase-3 were assayed by western blotting. Result： In comparison of the five other groups with model group, Sheng-huang mixture can decrease the myocardial apoptosis index and the protein expressions of Bax and Caspase-3, but increase the protein expressions of Bcl-2 in rats and the ratio of Bcl-2/Bax. Among which high dose Sheng-huang mixtrue group decreased much more than other groups. Conclusion： Sheng-huang mixture has the protective effects on myocardial ischemia-reperfusion injury in rats, which can inhibit the myocardial apoptosis of myocardial ischemia-reperfusion injury by increasing the protein expressions of Bcl-2 and reducing the protein expressions of Bax and Caspase-3.

关 键 词：生黄合剂 心肌缺血再灌注损伤 细胞凋亡 BAX蛋白 Bcl-2蛋白 CASPASE-3蛋白 

分 类 号：R285.5[医药卫生—中药学]