地特胰岛素对乳腺癌MCF-7和MDA-MB-231细胞生长的影响研究  

Effects of Insulin Detemir on the Growth of Breast Cancer Cells MCF-7 and MDA-MB-231

作　　者：汤喻[1] 张晓娟[1] 王守俊[1] 

机构地区：[1]郑州大学第一附属医院内分泌科,河南省郑州市450052

出　　处：《中国全科医学》2012年第18期2045-2048,共4页Chinese General Practice

摘　　要：目的探讨地特胰岛素对人乳腺癌MCF-7和MDA-MB-231细胞生长的影响及涉及的信号通路。方法地特胰岛素不同浓度、时间作用MCF-7和MDA-MB-231细胞,MTT法检测细胞数目,Western blot法检测细胞外信号调节蛋白激酶1/2(Erk1/2)和蛋白激酶B(Akt)磷酸化程度,流式细胞仪检测细胞周期。结果与正常对照相比,地特胰岛素1 U/L的地特胰岛素作用24 h,抑制MCF-7和MDA-MB-231细胞增殖不明显,1、10、100、1 000 U/L的地特胰岛素作用48 h和1 U/L的地特胰岛素作用48、72、96 h,促进两种细胞增殖的作用不明显,差异均无统计学意义(P>0.05)。10 U/L地特胰岛素作用MCF-7和MDA-MB-231细胞24 h细胞周期变化,Erk1/2和Akt磷酸化程度较正常对照组差异均无统计学意义(P>0.05)。结论地特胰岛素对MCF-7和MDA-MB-231细胞增殖作用不明显,Erk1/2和Akt的磷酸化程度无明显升高。Objective To investigate the effects of insulin Detemir on the growth of breast cancer cells MCF -7 and MDA -MB -231 and involved cell signaling pathways. Methods The breast cancer cells MCF -7 and MDA - MB -231 were treated by insulin Detemir with different concentration and action time. Cell proliferation was studied by MTT assay, phosphoryla- tion of Akt and Erkl/2 were evaluated by western blot and cell cycle was measured by flow eytometry. Results Different concen- tration of insulin Determir showed no statistically significant difference on the growth of MCF -7 and MDA - MB -231 ceils （P 〉 0. 05 ） . Different action time of insulin Determir also showed no statistically significant difference on the growth of MCF - 7 and MDA - MB -231 cells （P 〉 0.05 ） . Compared with the control group, the 24 h cell cycle, phosphorylation of Erk1/2 and Akt of MCF - 7 and MDA - MB - 231 cells showed no statistically significant difference （ P 〉 0. 05 ） . Conclusion Insulin Detemir cannot stimulate the proliferation of MCF- 7 and MDA -MB -231 cells significantly. The phosphorylation of Akt and Erkl/2 are not significantly increased.

关 键 词：地特胰岛素 乳腺肿瘤 丝裂原活化蛋白激酶 蛋白激酶B 

分 类 号：R737.9[医药卫生—肿瘤]