联合检测血清GP73和AFP对原发性肝癌诊断价值的探讨  

Diagnostic value of serum GP73 and AFP detection in primary hepatic carcinoma

作　　者：贾保昌[1] 罗小玲[1] 梁嵘[1] 岳惠芬[1] 葛莲英[1] 袁卫平[1] 沈筱芸[1] 

机构地区：[1]广西医科大学附属肿瘤医院肿瘤实验研究部,广西南宁530021

出　　处：《中华肿瘤防治杂志》2012年第11期832-835,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：广西自然科学基金(0832235);广西科技攻关项目(0816004-13;1140003A-32);广西医疗卫生重点课题(2011085)

摘　　要：目的:探讨血清高尔基体糖蛋白(GP73)对原发性肝癌的诊断价值,分析GP73和甲胎蛋白(AFP)联合检测对原发性肝癌诊断的临床意义。方法:应用双抗体夹心酶联免疫定量测定方法,检测144例原发性肝细胞癌、50例肝硬化、100例乙型肝炎、50例其他恶性肿瘤、26例肝良性肿瘤、84例HBV携带者和50名健康体检者血清中GP73的浓度,应用临床电化学发光法测定AFP作为参照。结果:原发性肝细胞癌患者血清GP73水平明显高于肝硬化、乙型肝炎、其他恶性肿瘤、肝良性肿瘤、HBV携带者和正常人对照组,P<0.05。通过ROC曲线设定GP73浓度,GP73为64ng/mL作为肝癌诊断的临界值切点(cut-off)时,GP73检测原发性肝细胞癌的敏感度和特异度达到最高,分别为83.3%和88.3%。在原发性肝细胞癌组,GP73诊断肝癌的敏感度和特异度与AFP比较差异有统计学意义(83.3%vs 72.2%,88.3%vs 76.7%),P<0.05;GP73+AFP联合检测可使敏感性提高到94.4%,特异性为65.6%。结论:GP73诊断原发性肝细胞癌有较好的敏感性和特异性,而GP73与AFP联合检测的敏感性比单项检测均高。联合检测血清中的GP73和AFP可提高原发性肝细胞癌的诊断价值。OBJECTIVE：To explore the diagnostic value of serum GP73 in the patient of primary hepatic carcinoma （PHC） ,and discuss the diagnostic value of the joint roles of serum GP73 and AFP in the diagnosis of PHC. METHODS： ELISA method was used to detect the serum level of GP73 in 144 cases of PHC, 50 cases of hepatic cirrhosis, 100 cases of hepatitis, 50 cases of other malignant tumors, 26 cases of liver benign tumor, 84 cases of hepatitis carriers and 50 cases of healthy people. The electrochemical luminescence method was used to detect the serum level of AFP of the all, and the lev- el of AFP was as a comparison. RESULTS： The level of serum GP73 in the group of PHC were significantly higher than that of hepatic cirrhosis, hepatitis, other malignant tumors, liver benign tumor, hepatitis carriers and healthy people （P〈 0.05）, the critical value for GP73 was determined to be 64.4 ng/mL through the ROC curves, under the critical value the sensitivity and specificity of the GP73 was 83.3% and 88.3% ,and these were the best. The difference of GP73 and AFP in the group of PHC was statistically significant （83.3% vs 72.2%0,88.3% vs 76.7% ,P〈0. 05） ,the diagnostic sensitivi- ty of the joint roles of serum GP73 and AFP was increased to 94.4%, and the specificity was 65.6 %. CONCLUSIONS： The GP73 has better sensitivity and specificity than AFP. The sensitivity of the joint test is better than the single detec- tion. The joint roles of serum GP73 and AFP ean improve PHC diagnostic value.

关 键 词：肝肿瘤 原发 肝肿瘤/诊断 高尔基蛋白73 甲胎蛋白 

分 类 号：R735.7[医药卫生—肿瘤]