肾癌组织Pokemon基因表达及其生物学意义的研究  

Expression of proto-oncogene Pokemon and its biological significance in renal cell carcinoma tissues

作　　者：侯列军[1] 郑军华[2] 耿江[2] 顾闻宇[2] 王波[2] 

机构地区：[1]宁波大学医学院附属医院泌尿外科,浙江宁波315020 [2]同济大学附属上海市第十人民医院泌尿外科,上海200072

出　　处：《中华肿瘤防治杂志》2012年第11期836-839,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨Pokemon基因在肾细胞癌组织中的表达及其生物学意义。方法:收集47例肾癌手术切除的癌、癌旁及邻近正常组织,用逆转录实时聚合酶链反应(Real-time PCR)及蛋白质印迹法分别从核酸及蛋白水平检测标本中Pokemon基因的表达,并结合性别、年龄、肿瘤大小、病理分级和TNM分期等临床资料进行统计分析,对mRNA和蛋白表达的水平进行相关性研究。结果:Real-time PCR及蛋白质印迹法均检测出Pokemon在肾癌、癌旁及正常对照组织中的表达,差异有统计学意义,P<0.05。肾癌组织中,Pokemon的表达与肿瘤病理分级有关,而与患者的性别、年龄、肿瘤最大直径、TNM及病理类型无明显相关性。肾组织中,Pokemon的mRNA与蛋白的表达呈正相关,r=0.494,P<0.05。结论:Pokemon表达和肾癌的发病及恶性程度有关,有望成为肾癌的预后指标和肾癌靶向治疗的有效靶点。OBJECTIVE： To explore the expression of Pokemon in human renal cell carcinoma and its correlation with clinical features. METHODS： Reverse transcription real-time PCR and Western blot were applied to detect the ex- pression of Pokemon in 47 cases of resection samples, their corresponding paraeancerous samples and normal samples. Cor- relation between expression of Pokemon and clinical features such as gender, age, tumor size, tumor differentiation degree and TNM stages was analyzed. RESULTS： The expression of Pokemon mRNA and protein were both significantly higher in the cancerous tissues than those in the paracancerous tissues and normal tissues （P^0.05）. The expression of Pokemon was related to tumor differentiation degree, but had no correlation with patient＇s gender, age, maximum diameter of tumor, pathological type and TNM stages. Expression of Pokemon mRNA was positively correlated with protein, the cor- relation coefficient was 0. 494（P〈0.05）. CONCLUSIONS： Pokemon is expressed in human renal cell carcinoma and it may play a key role in the genesis and malignancy of renal cell carcinoma. It may be a prognostic factor and therapeutic target of renal cell carcinoma in the future.

关 键 词：癌 肾细胞 肾肿瘤 基因 Pokemon印迹法 蛋白质 

分 类 号：R737.11[医药卫生—肿瘤]