宫颈病变组织CK17蛋白表达临床意义的研究  

Expression and clinical significance of Ck17 in cervical lesion tissues

作　　者：王为民[1] 谢朋木[1] 许艳梅[2] 侯洪春[3] 

机构地区：[1]单县中心医院妇产科,山东单县274300 [2]单县中心医院病理科,山东单县274300 [3]济南市妇幼保健院妇科,山东济南250001

出　　处：《中华肿瘤防治杂志》2012年第11期848-850,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:检测宫颈上皮内瘤变及宫颈癌组织中CK17的表达,并探讨其临床意义。方法:应用免疫组织化学SP法,检测15例正常宫颈组织、15例宫颈上皮内瘤变(CIN)Ⅰ、17例CINⅡ、21例CINⅢ、36例宫颈鳞癌和8例宫颈腺癌组织中CK17的表达情况。结果:CK17在正常宫颈组织、CIN和宫颈鳞癌中的阳性表达率分别为0、56.6%和83.3%,3组相比差异有统计学意义,χ2=30.177,P<0.05。CINⅠ、CINⅡ和CINⅢ中,CK17的阳性表达率分别为20.0%、64.7%和76.2%,CINⅡ组阳性率高于CINⅠ组,差异有统计学意义,P=0.016;CINⅢ组阳性率高于CINⅠ组,差异有统计学意义,P=0.002;CINⅡ组和CINⅢ组阳性率差异无统计学意义,P>0.05。随着宫颈病变程度的加重,CK17的阳性表达率逐渐增高,其阳性表达同宫颈病变程度呈正相关,r=0.958,P<0.05。结论:CK17可作为高级别CIN和浸润性鳞状细胞癌诊断及预测病变进展趋势的标志。OBJECTIVE：To detect the expression and study the clinical significance of CK17 protein in cervical intra- epithelial neoplasia （CIN） and cervical cancer samples. METHODS： The SP method of immunochemistry was employed to investigate the expression of CK17 in all specimens, including 15 cases of normal cervix epithelia, 53 cases of cervical intra- epithelial neoplasia（CIN Ⅰ 15,CIN Ⅱ 17,CIN Ⅲ 21） and 44 cases of cervical cancer. RESULTS： CK17 staining was iden- tified in 56.6% of CIN and 83.3 % of cervical squamous cell carcinoma cases but was completely absent in reference cervix epithelium. The difference among the three groups was statistically significant （X2 = 30. 177, P（0.05）. In CIN group, expression rates were 20.0%, 64.7 % and 76.2%, which was significantly higher in CIN Ⅱ and CIN Ⅲ compared with CIN I （P=0. 016;P=0. 002） ,but the statistically significant difference was not found between CIN Ⅱ and CINⅢ （P〈0.05）. Positivity for CKI7 correlated with increasing lesion grade of the intraepithelial lesions, the extent of the CK17-positive rate and the grade of the intraepithelial lesions have a positive correlation （r=0. 958 ,P〈0.05）. CONCLUSIONS： CK17 is a valuable marker for monitoring of disease progression in human cervix and the diagnosis of CIN and cervical squamous cell carcinoma.

关 键 词：宫颈上皮内瘤变 宫颈肿瘤 CK17 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]