Determination of zidovudine using anion exchange chromatography with integrated pulsed amperometric detection  

作　　者：Yu Chen Yu Xiu Liu Zhi Dong Chen Mei Lan Chen Yan Zhu 

机构地区：[1]Hangzhou Vocational and Technical College, Hangzhou 310018, China [2]School of Chemistry and Chemical Engineering, Changzhou University, Changzhou 213164, China [3]College of Biology and Envinonment Engineering. Zhejiang Shuren University, l-langzhou 310015, China [4]Department of Chemistry, Zhejiang University, Hangzhou 310028, China

出　　处：《Chinese Chemical Letters》2012年第6期715-718,共4页中国化学快报（英文版）

基　　金：supported by Zhejiang Provincial Natural Science Foundation of China(No.Y2110945);Zhejiang Provincial Analysis and Testing Foundation of China(No.2011C37065)

摘　　要：A rapid and practical method for direct detection of zidovudine in high performance anion exchange chromatography （HPAEC） has been developed with integrated pulsed amperometric detection （IPAD）. Dionex AS18 （250mm × 2mm） and AG18 （50 mm× 2 mm） columns and 11 mmol/L NaOH solution were used for separation. Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio （S/N）. Utilizing an optimized waveform, the repeatability （intra-day） precision and intermediate （inter-day） precision are obtained with relative standard deviation （RSD） of 1.88, 2.27, respectively. The limit of quantification （LOQ） and limit of detection （LOD） were found to be 9.70, 3.0 ng/mL, respectively, with correlation coefficients of 0.9992 over concentration range 0.01-10 μg/mL. The present method was successfully applied to the determination of zidovudine in human plasma. The recoveries of plasma sample spiked by 0.7 μg/mL, 2.7 μg/mL obtained were 95.3-101.5%, with relative standard deviation （RSD） of 2.54%, 2.21%, respectively.A rapid and practical method for direct detection of zidovudine in high performance anion exchange chromatography （HPAEC） has been developed with integrated pulsed amperometric detection （IPAD）. Dionex AS18 （250mm × 2mm） and AG18 （50 mm× 2 mm） columns and 11 mmol/L NaOH solution were used for separation. Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio （S/N）. Utilizing an optimized waveform, the repeatability （intra-day） precision and intermediate （inter-day） precision are obtained with relative standard deviation （RSD） of 1.88, 2.27, respectively. The limit of quantification （LOQ） and limit of detection （LOD） were found to be 9.70, 3.0 ng/mL, respectively, with correlation coefficients of 0.9992 over concentration range 0.01-10 μg/mL. The present method was successfully applied to the determination of zidovudine in human plasma. The recoveries of plasma sample spiked by 0.7 μg/mL, 2.7 μg/mL obtained were 95.3-101.5%, with relative standard deviation （RSD） of 2.54%, 2.21%, respectively.

关 键 词：ZIDOVUDINE AIDS Integrated pulsed amperometry High performance anion exchange chromatography PLASMA 

分 类 号：TN958.98[电子电信—信号与信息处理] TH832[电子电信—信息与通信工程]