Development of a monolithic polymer pipette for solid-phase extraction of liquiritigenin in rat plasma  

作　　者：Hong Wu Zhang Kang Li Zhi Xian Liang Feng YangWang Qi Wen Lu 

机构地区：[1]School of Pharmacy, Guangdong Pharmaceutical College, Guangzhou 510006, China

出　　处：《Chinese Chemical Letters》2012年第6期723-726,共4页中国化学快报（英文版）

基　　金：supports of the National Natural Science Foundation of China (No.30801515);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry(No.[2011]1139);the Open Fund of Key Laboratory of Analytical Chemistry for Living Biosystems, CAS(No.ACL201202)

摘　　要：A monolithic polymer column with mixed-mode interaction was prepared by in situ polymerization in a 1000μL pipette. Two kinds of monomers, butyl methacrylate （BMA） and 2-（dimethylamino）ethyl methacrylate （DMAM） were applied to constructing the mixed-mode interaction of monolithic polymer column. Its solid-phase extraction properties for liquiritigenin （LQG） were evaluated by high performance liquid chromatography （HPLC） with a gradient elution procedure. After the extraction procedure was optimized, the maximum binding capacity and extraction recovery following the optimal extraction procedure were investigated. Calibration curve was expressed as A = 65.9C ＋ 4.53 （r2 = 0.998） with a linear range of 0.151-1.80μg/mL. The experimental results indicate that the monolithic polymer pipette presents good extraction efficiency for LQG. It can be envisaged that the developed monolithic polymer pipette possesses the potential for its application to the enrichment of other flavonoids compounds being similar to the structure of LQG.A monolithic polymer column with mixed-mode interaction was prepared by in situ polymerization in a 1000μL pipette. Two kinds of monomers, butyl methacrylate （BMA） and 2-（dimethylamino）ethyl methacrylate （DMAM） were applied to constructing the mixed-mode interaction of monolithic polymer column. Its solid-phase extraction properties for liquiritigenin （LQG） were evaluated by high performance liquid chromatography （HPLC） with a gradient elution procedure. After the extraction procedure was optimized, the maximum binding capacity and extraction recovery following the optimal extraction procedure were investigated. Calibration curve was expressed as A = 65.9C ＋ 4.53 （r2 = 0.998） with a linear range of 0.151-1.80μg/mL. The experimental results indicate that the monolithic polymer pipette presents good extraction efficiency for LQG. It can be envisaged that the developed monolithic polymer pipette possesses the potential for its application to the enrichment of other flavonoids compounds being similar to the structure of LQG.

关 键 词：Monolithic polymer pipette Solid phase extraction High performance liquid chromatography LIQUIRITIGENIN 

分 类 号：TQ225.241[化学工程—有机化工] TE357.46[石油与天然气工程—油气田开发工程]