Arabidopsis F-box gene FOA1 involved in ABA signaling  

作　　者：PENG Juan YU DaShi WANG LiQun XIE MinMin YUAN CongYing WANG Yu TANG DongYing ZHAO XiaoYing LIU XuanMing 

机构地区：[1]College of Life Sciences,State Key Laboratory of Chemo/Biosensing and Chemometrics,Hunan University,Changsha 410082,China

出　　处：《Science China(Life Sciences)》2012年第6期497-506,共10页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.31171176);Research Fund for the Doctoral Program of Higher Education of China(Grant No.755228001);Natural Science Foundation of Hunan Province(Grant No.11JJA002);the National Key Laboratory of Plant Molecular Genetics(Grant No.Y109Z711T1)

摘　　要：The expression of FOAl （F-box overexpressed/oppressed ABA signaling） in different organs of Arabidopsis, and in response to ABA and NaCI, was analyzed. The expression level of FOAl is higher in the root and is lower in the stem, and is induced rapidly by ABA and NaC1. The phenotypes of T-DNA insertion mutant foal and FOAl overexpression lines FOAloxl and FOAlox2 were analyzed. The foal mutant exhibited a lower germination rate, shorter root length, more stomatal opening, in- creased proline accumulation and hypersensitivity to ABA compared with the wild type. In contrast, the overexpression lines showed lower sensitivity to ABA than the wild type. The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foal mutant in response to ABA. Compared with the wild type, the expression levels of ABA-responsive transcription factors were higher, but ABA and stress-responsive genes were lower in foal mutant. This study demonstrates that FOAl is an ABA signaling-related gene, and may play a negative role in ABA signaling.The expression of FOA1(F-box overexpressed/oppressed ABA signaling) in different organs of Arabidopsis,and in response to ABA and NaCl,was analyzed.The expression level of FOA1 is higher in the root and is lower in the stem,and is induced rapidly by ABA and NaCl.The phenotypes of T-DNA insertion mutant foa1 and FOA1 overexpression lines FOA1ox1 and FOA1ox2 were analyzed.The foa1 mutant exhibited a lower germination rate,shorter root length,more stomatal opening,increased proline accumulation and hypersensitivity to ABA compared with the wild type.In contrast,the overexpression lines showed lower sensitivity to ABA than the wild type.The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foa1 mutant in response to ABA.Compared with the wild type,the expression levels of ABA-responsive transcription factors were higher,but ABA and stress-responsive genes were lower in foa1 mutant.This study demonstrates that FOA1 is an ABA signaling-related gene,and may play a negative role in ABA signaling.

关 键 词：F-box gene FOAL ABA SIGNALING ARABIDOPSIS 

分 类 号：Q111[生物学—普通生物学] Q945.78