丁酸钠对抗内毒素血症小鼠肺损伤的保护作用  

Protective effects of sodium butyrate against lung injury in mice with endotoxemia

作　　者：曾韬[1] 张力[2] 

机构地区：[1]荆楚理工学院医学院,荆门448000 [2]重庆医科大学病理生理学教研室,重庆400016

出　　处：《生理学报》2012年第3期308-312,共5页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China (No. 30900651);the Science Research Project of Chongqing Municipal Education Commission;China (No. KJ090304)

摘　　要：本文旨在研究丁酸钠对内毒素血症小鼠体内炎症反应、肺组织损伤及生存率的影响。Balb/c小鼠经腹腔注射丁酸钠(200 mg/kg)后,予以致死剂量脂多糖(20 mg/kg, i.p.)以诱导严重内毒素血症。造模后观察小鼠存活时间或在18 h后处死小鼠,收集血浆检测细胞因子TNF-α和IL-6水平;取肺组织检测肺组织湿/干重比、髓过氧化物酶活性,并经石蜡切片HE染色观察组织病理学改变。结果显示,丁酸钠明显减轻内毒素血症小鼠肺组织病理学损伤,降低肺组织湿/干重比及髓过氧化物酶活性。丁酸钠还可显著降低内毒素血症小鼠血浆TNF-α和IL-6水平,并提高实验动物生存率。以上结果提示,丁酸钠可有效抑制内毒素血症小鼠肺内炎症反应,提高内毒素血症小鼠生存率。The aim of the present study was to investigate the effects of sodium butyrate（SB） on systemic inflammation,lung injury and survival rate of mice with endotoxemia.Balb/c mice were pre-treated with SB or vehicle,and then endotoxemia was induced by lethal dose of lipopolysaccharide（LPS,20 mg/kg,i.p.） and the survival rate of mice was monitored.A separated set of animals were sacrificed at 18 h after LPS challenge,and blood samples were harvested for measuring TNF-α and IL-6 levels.Lung tissues were also harvested to determine the ratio of wet weight to dry weight of lung tissue and myeloperoxidase（MPO） activity in lung tissue.In addition,the formalin-fixed lung specimens were stained with HE routinely for morphologic evaluation.The results showed that pretreatment with SB alleviated LPS-induced morphological damage in lung tissue.This was accompanied by reduced ratio of wet weight to dry weight of lung tissue and MPO activity in lung homogenates.Additionally,the up-regulation of pro-inflammatory cytokines TNF-α and IL-6 was also suppressed by SB,while the survival rate of mice with lethal endotoxemia was significantly increased by SB pre-treatment.The results suggest that SB effectively attenuates intrapulmonary inflammatory response and improves the survival of endotoxemic mice.

关 键 词：组蛋白去乙酰化酶 内毒素血症 肺损伤 炎症 

分 类 号：R363[医药卫生—病理学]