去铁胺对大鼠缺血心肌微血管形成的影响及可能机制  

Effect and possible mechanism of desferrioxamine on microvascular formation of ischemic myocardium in rats

作　　者：黄文新[1] 张敏娜[1] 林海星[1] 

机构地区：[1]桂林医学院附属医院心血管病科,广西省541001

出　　处：《江苏医药》2012年第12期1370-1372,共3页Jiangsu Medical Journal

基　　金：广西自然科学基金资助(0991257)

摘　　要：目的探讨去铁胺(DFO)对大鼠缺血心肌微血管形成的作用及可能机制。方法 48只SD大鼠随机均分为正常对照组(A组)、假手术组(B组)、结扎组(C组)和DFO 200mg/kg组(D组)。术后7d,处死大鼠,免疫组化法检测微血管密度(MVD)及低氧诱导因子1α(HIF-1α)蛋白表达,RT-PCR检测HIF-1αmRNA表达。结果与A、B组相比,C、D组MVD、HIF-1α蛋白和mRNA表达均增加(P<0.05或P<0.01),其中D组各指标增加更为显著(P<0.01)。结论 DFO可以促进大鼠缺血心肌组织微血管形成;其作用机制可能与上调HIF-1α的表达有关。Objective To investigate the effect and possible mechanism of deferoxamine（DFO） on microvascular formation of ischemic myoeardium in rats. Methods Forty-eight SD rats were equally randomized into four groups of A （ normal controls）, B （ sham operated）, C （ligating the left anterior descending of the coronary artery） and D（injeeted with DFO 200 mg/kg before the ligation）. SD rats were sacrificed 7 days later. Mierovessel density（MVD） and the protein expression of hypoxia- inducible factor-la（HIF-la） were detected by immunohistoehemistry, and the mRNA expression of HIF-la was measured by RT-PCR. Results Compared with groups of A and B,MVD and the protein and mRNA expressions of HIF-la were all increased in groups of C and D（P〈0. 05 or P〈0. 01） ,the changes of which were more significant in group D（P〈0. 01）. Conclusion Deferoxamine can promote the microvaseular formation of ischemic myocardium in rats, probably via up-regulating HIF-1a expression.

关 键 词：去铁胺 急性心肌缺血 低氧诱导因子1Α 微血管密度 

分 类 号：R363[医药卫生—病理学]