辽宁丹东间日疟原虫裂殖子表面蛋白1等位基因型分析  

Allele Genetype Analysis of Plasmodium vivax Merozoite Surface Protein1 (PvMSP-1) from Dangdong,Liaoning Province

作　　者：耿英芝[1] 毛玲玲[1] 腾聪[1] 安春丽[2] 王博[1] 陈静乙[2] 姚文清[1] 

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005 [2]中国医科大学,沈阳110001

出　　处：《中国寄生虫学与寄生虫病杂志》2012年第3期242-244,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家科技重大专项(No.2009ZX10004-209)~~

摘　　要：用套式PCR方法扩增辽宁丹东地区间日疟原虫裂殖子表面蛋白1(MSP-1)基因ICB5-ICB6片段,经PvuⅡ酶切和琼脂糖凝胶电泳,对产物进行序列分析。结果显示,11份镜检确诊的间日疟患者血液标本经套式PCR扩增均出现大小约为470 bp(Sal-1型)或400 bp(Belem型)的特异性片段。经PvuⅡ内切酶消化后,其中5份血样(470 bp),出现120和350 bp酶切片段,为Sal-1型;其余6份血样(400 bp)中,1份出现400 bp片段,为Belem型,1份出现120和280 bp两种酶切片段,为重组Ⅲ型,4份出现120和240 bp两种酶切片段,为朝鲜型。辽宁省丹东地区的间日疟原虫PvMSP-1基因存在3种不同的等位基因型,以Sal-1型和朝鲜型为主,但无不同等位基因型的混合感染。Nested PCR method was used to amplify the Plasmodium vivax merozoite surface protein 1 （PvMSP-1） gene fragment containing the ICB5 and ICB6 region from Plasmodium vivax in Liaoning Province. The PCR products were digested by Pvu Ⅱ restriction endonuclease and the digested fragments were observed by 1.5% agarose gel electrophoresis, and all followed by sequencing analysis and comparion. In 11 field isolates of P. vivax, two kinds of DNA fragments with 470 and 400 bp were produced respectively. After PvulI digestion, two Sal-1 type fragments （120 and 350 bp） were ob- tained from 5 samples of 470 bp. Single band of 400 bp appeared in 1 samples as Belem type. Two bands of 120 and 280 bp appeared from another 1 sample as recombination type III, and other 4 bands with 120 and 240 bp as Korean iso- late. The principal types of PvMSP-1 alleles exist in malaria endemic areas in Liaoning Province with no mixed infection of two different type alleles.

关 键 词：间日疟原虫 裂殖子表面蛋白1 等位基因 序列分析 

分 类 号：R382.3[医药卫生—医学寄生虫学]