环杷明对人腮腺多形性腺瘤细胞凋亡的影响  

Cyclopamine Induces Apoptosis in Human Salivary Pleomorphic Adenoma Cells

作　　者：欧学平[1] 肖翠翠[1] 宋萌[1] 肖忠明[2] 韦芳[2] 洪艳[2] 

机构地区：[1]上海交通大学附属上海第一人民医院口腔科,上海200080 [2]上海交通大学附属上海第一人民医院实验中心,上海200080

出　　处：《口腔颌面外科杂志》2012年第3期163-167,共5页Journal of Oral and Maxillofacial Surgery

基　　金：上海市科学技术委员会资助项目(08JC1412900);上海市重点学科建设项目(S30206-KF05)

摘　　要：目的:探讨环杷明(cyclopamine)诱导人腮腺多形性腺瘤细胞凋亡的作用及其对Gli-2和Bcl-2表达的影响。方法:用10μmol∕L环杷明处理人腮腺多形性腺瘤细胞,48 h后倒置显微镜下观察细胞数量及形态变化;48 h后实时定量PCR检测实验组(环杷明组)和空白对照组、DMSO对照组的Gli-2、Bcl-2 mRNA表达水平差异;24 h后流式细胞术观察细胞凋亡。结果:人腮腺多形性腺瘤细胞用10μmol∕L环杷明作用48 h后倒置显微镜下可见细胞数量减少、细胞核固缩或碎裂、核仁变形等典型的细胞凋亡形态学变化;环杷明组与空白对照组、DMSO对照组相比,可以显著下调Gli-2、Bcl-2 mRNA表达(P<0.01);环杷明组的细胞凋亡率明显高于空白对照组和DMSO对照组(P<0.01)。结论:环杷明可诱导人腮腺多形性腺瘤细胞凋亡,其作用机制可能与下调Gli-2、Bcl-2 mRNA表达,活化细胞凋亡的线粒体途径有关。Objective： The purpose of this study was to examine whether cyclopamine is related to apoptosis and growth inhibition of human salivary pleomorphic adenoma （HSPA） cells. Methods： HSPA cells were treated with cyclopamine. Observed the cells quantity change and morphology after 48 h. Cells cultured with DMSO 0.08% or void were used as control. Gli-2 and Bcl-2 mRNA expression was examined by Real-time PCR after 48 h. Flow eytometry was used to analyze cell apoptosis after 24 h. Experiment results are presented as x^-±s. Statistical analysis was undertaken with SAS 9.13. The differences among the groups were identified by one-way ANOVA. Significance was accepted at P〈0.01. Result： The number of eyclopamine treated HSPA cells was significantly decreased. Cyclopamine treated （10 μmol/L） HSPA cells showed nuclear psychosis or fragmentation, chromatospherite disfiguration with apoptoticmorphology. The expression levels of Gli-2 and Bel-2 mRNA in 10/J, mol / L cyelopamine treated group was down-regulated significantly compare with blank and DMSO control groups （P〈0.01）. Apoptosis rate of 10μ mol / L cyclopamine treatment group was significantly higher than blank and DMSO control group （P〈0.01）. Conclusion： Cyclopamine appears to induce growth inhibition and apoptosis in human salivary pleomorphie adenoma cells. The mechanism may be related to down-regulation of Gli-2 and Bcl-2 mRNA, which activates the mitochondrial apoptotic pathways.

关 键 词：腮腺多形性腺瘤细胞 细胞凋亡 环杷明 

分 类 号：R739.87[医药卫生—肿瘤]