Response to weaning and dietary L-glutamine supplementation:metabolomic analysis in piglets by gas chromatography/mass spectrometry  

作　　者：Ying-ping XIAO Tian-xing WU Qi-hua HONG Jiang-ming SUN An-guo CHEN Cai-mei YANG Xiao-yan LI 

机构地区：[1]College of Animal Sciences,Zhejiang University,Hangzhou 310058,China [2]Key Laboratory,for Molecular Design and Nutrition Engineering,Ningbo Institute of Technology,Zhejiang University,Ningbo 315100,China [3]Department of Chemistry,,Tongji University,Shanghai 200092,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2012年第7期567-578,共12页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China(No.30700578);the Local Cooperative Enterprises of Zhejiang Province,China(Nos.H20080692 and H20020883)

摘　　要：A novel metabolomic method based on gas chromatography/mass spectrometry （GC-MS） was applied to determine the metabolites in the serum of piglets in response to weaning and dietary L-glutamine （Gin） supplementation. Thirty-six 21-d-old piglets were randomly assigned into three groups. One group continued to suckle from the sows （suckling group）, whereas the other two groups were weaned and their diets were supplemented with 1% （w/w） Gin or isonitrogenous L-alanine, respectively, representing Gin group or control group. Serum samples were collected to characterize metabolites after a 7-d treatment. Results showed that twenty metabolites were down-regulated significantly （P〈0.05） in control piglets compared with suckling ones. These data demonstrated that early weaning causes a wide range of metabolic changes across arginine and proline metabolism, aminosugar and nucleotide metabolism, galactose metabolism, glycerophospholipid metabolism, biosynthesis of unsaturated fatty acid, and fatty acid metabolism. Dietary Gin supplementation increased the levels of creatinine, D-xylose, 2-hydroxybutyric acid, palmitelaidic acid, and a-L-galactofuranose （P〈0.05） in eady weaned piglets, and were involved in the arginine and proline metabolism, carbohydrate metabolism, and fatty acid metabolism. A leave-one-out cross-validation of random forest analysis indicated that creatinine was the most important metabolite among the three groups. Notably, the concentration of creatinine in control piglets was decreased （P=0.00001） compared to the suckling piglets, and increased （P=0.0003） in Gin-supplemented piglets. A correlation network for weaned and suckling piglets revealed that early weaning changed the metabolic pathways, leading to the abnormality of carbohydrate metabolism, amino acid metabolism, and lipid metabolism, which could be partially improved by dietary Gin supplementation. These findings provide fresh insight into the complex metabolic changes in response to early weaning and dietary A novel metabolomic method based on gas chromatography/mass spectrometry(GC-MS)was applied to determine the metabolites in the serum of piglets in response to weaning and dietary L-glutamine(Gln)supplementation.Thirty-six 21-d-old piglets were randomly assigned into three groups.One group continued to suckle from the sows(suckling group),whereas the other two groups were weaned and their diets were supplemented with 1%(w/w)Gln or isonitrogenous L-alanine,respectively,representing Gln group or control group.Serum samples were collected to characterize metabolites after a 7-d treatment.Results showed that twenty metabolites were down-regulated significantly(P<0.05)in control piglets compared with suckling ones.These data demonstrated that early weaning causes a wide range of metabolic changes across arginine and proline metabolism,aminosugar and nucleotide metabolism,galactose metabolism,glycerophospholipid metabolism,biosynthesis of unsaturated fatty acid,and fatty acid metabolism.Dietary Gln supplementation increased the levels of creatinine,D-xylose,2-hydroxybutyric acid,palmitelaidic acid,andα-L-galactofuranose(P<0.05)in early weaned piglets,and were involved in the arginine and proline metabolism,carbohydrate metabolism,and fatty acid metabolism.A leave-one-out cross-validation of random forest analysis indicated that creatinine was the most important metabolite among the three groups.Notably,the concentration of creatinine in control piglets was decreased(P=0.00001)compared to the suckling piglets,and increased(P=0.0003)in Gln-supplemented piglets.A correlation network for weaned and suckling piglets revealed that early weaning changed the metabolic pathways,leading to the abnormality of carbohydrate metabolism,amino acid metabolism,and lipid metabolism,which could be partially improved by dietary Gln supplementation.These findings provide fresh insight into the complex metabolic changes in response to early weaning and dietary Gln supplementation in piglets.

关 键 词：PIGLET Metabolomics WEANING GLUTAMINE Gas chromatography/mass spectrometry 

分 类 号：S811.3[农业科学—畜牧学]