HPV病毒负荷量与TSLC-1在宫颈鳞癌及癌前病变中的相关性研究  

The Relationship Between the Viral Load of HPV and TSLC-1 in the Cervical Squamous Cell Carcinoma and Cervical Lesions

作　　者：高丽[1] 杨永秀[1] 卢佳[1] 杨爱宏[1] 冯惠琴[1] 许飞雪[1] 

机构地区：[1]甘肃省兰州大学第一医院,730000

出　　处：《国际妇产科学杂志》2012年第3期294-296,共3页Journal of International Obstetrics and Gynecology

摘　　要：目的:探讨肺癌肿瘤抑制因子1(TSLC-1)与人乳头瘤病毒(HPV)病毒负荷量在癌及癌前病变中的相关性。方法:收集2008—2010年就诊于兰州大学第一医院100例慢性宫颈炎、宫颈上皮内瘤变及宫颈鳞状上皮癌患者的宫颈脱落上皮细胞,采用杂交捕获法(HC-Ⅱ)检测HPV病毒负荷量,免疫组化(SP)法检测相应组织TSLC-1蛋白表达,分析两者在宫颈病变进展中的关系。结果:100例标本HPV阳性54例,1≤病毒负荷量(RLU/CO)≤100共27例,100～1 000共23例,≥1 000为4例。TSLC-1蛋白阴性为42例,阳性58例,按不同表达强度分别为"+"23例,"++"20例,"+++"15例。Spearman秩相关分析显示,HPV病毒负荷量与TSLC-1表达有相关性(rs=0.586,P=0.000)。结论:随宫颈癌前病变进展至宫颈鳞癌,HPV病毒负荷量与TSLC-1表达减低有相关性,可将两者联合检测应用于临床,预测宫颈癌前病变的进展。Objective：To study the relationship of the viral load of HPV and TSLC-1 during the development of cervical squamous cancer.Methods：100 specimens of tissues which had been detected the viral load of HPV by HC-Ⅱ,including squamous carcinoma of the cervix,cervical intraepithelial neoplasia（CINs） and chronic cervicitis underwent immunehistochemistry to detect the expression of TSLC-1,and evaluated their correlations.Results：54 out of 100 samples were HPV DNA positive,the viral load of HPV were 1≤RLU/CO≤100（27/100）,100-1 000（23/100）,≥1 000（4/100）,TSLC-1＂-＂（42/100）,＂＋＂（23/100）,＂＋＋＂（20/100） and＂＋＋＋＂（15/100）.Statistical analysis for the association of TSLC-1 and the viral load of HPV revealed that： the expression levels of TSLC-1 and the viral load of HPV showed a significant correlation（rs=0.586,P=0.000）.Conclusions：Down-regulation of TSLC-1 and the viral load of HPV showed a significant correlation during the pathogenesis of CIN and cervical squamous cell carcinoma,to detect both of them is helpful to calculated the tendency of cervical pathological changes.

关 键 词：基因 肿瘤抑制 病毒载量 宫颈肿瘤 癌 鳞状细胞 宫颈上皮内瘤样病变 乳头状瘤病毒科 

分 类 号：R737.33[医药卫生—肿瘤]