奥沙利铂与7-羟基星形孢菌素对结肠癌HCT116细胞的杀伤作用及其机制的研究  

Study on the lethal effect and mechanisms of oxaliplatin and UCN -O1 on HCT116 colon cancer cell line

作　　者：郑文鑫[1] 韩艳玲[1] 魏海波[1] 张晓金[1] 姜鹏宇[1] 吕雪莹[1] 

机构地区：[1]哈尔滨医科大学免疫学教研室哈尔滨150081

出　　处：《实用肿瘤学杂志》2012年第3期199-203,共5页Practical Oncology Journal

基　　金：教育部“春晖计划”（z2008-1-15031）;哈尔滨医科大学医学基础学科青年科学基金资助项目（060028）;国家自然科学基金青年基金资助项目（30700997）

摘　　要：目的奥沙利铂与7-羟基星形孢菌素单独及联合应用，探讨诱导结肠癌细胞HCTl16死亡的作用机制。方法应用MTT法检测L-OHP、UCN-01单药及联用对HCTll6细胞的增殖抑制作用；应用流式细胞术、Western blot方法检测药物处理后的结肠癌细胞凋亡情况；应用HE染色法检测丝裂灾变的形态改变。结果低浓度L-OHP（6．25pμmol／L）与UCN-01（100nmol／L）联用对结肠癌HCTll6细胞的杀伤可产生协同作用，效果明显强于单独用药组；两药联用AnnexinV阳性细胞率与UCN-01单用无明显差别，但死亡细胞明显增加；两药联用HCTll6细胞的形态发生改变，巨核多核细胞较单独用药组增多约10％。结论 L-OHP与UCN-01联用对HCTll6细胞具有协同抑制作用，诱导HCTll6细胞发生凋亡和丝裂灾变。Objective To observe the growth inhibitory effect of oxaliplatin （ L - OHP） and （7 - hydroxystaurosporine, UCN -01 ）on HCT116 colon cancer cell line in single or combination treatment, and to investigate its mechanisms. Method The growth inhibitory effect was evaluated by MTT assay in single agent or combination treatment. Apoptosis was investigated by flow cytometry and Western blotting, and mitotic eatastrophy was determined by HE staining. Results Combination treatment of L - OHP in low concentration and UCN -01 has synergistic effect on killing HCT116 colon cancer cell line, which was more effective than single treatment. AnnerxinV positive cell rate showed no difference between combination treatment and UCN -01 alone. Cellular morphology of HCT116 cell was changed in combination treatment, and megakaryocytes and polykaryocytes in combination treatment were increased by approximately 10% compared with the single treatment. Conclusion Combinition treatment of L- OHP and UCN -01 has synergistic inhibitory effect on HCT116 cell proliferation through inducing cell apoptosis and mitotic catastrophy.

关 键 词：奥沙利铂 UCN-01 增殖抑制 结肠癌细胞 HCT116 

分 类 号：R735.35[医药卫生—肿瘤]