分割剂量射线照射富集宫颈癌肿瘤干细胞的研究  

Fractionated-dose Irradiation Enriching Cervical Cancer Stem Cells In Vitro

作　　者：谢玲玲[1] 卢淮武[1] 钟沅月[1] 吴妙芳[1] 陆晓楣[1] 姚婷婷[1] 林仲秋[1] 

机构地区：[1]中山大学孙逸仙纪念医院妇产科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2012年第3期330-335,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30872743/C1701);广州市科技项目(2010J-E291)

摘　　要：【目的】研究分割剂量射线照射的宫颈癌Hela细胞表达肿瘤干细胞相关蛋白情况及裸鼠体内成瘤能力,探讨经此法富集宫颈癌肿瘤干细胞的可行性。【方法】Hela细胞分组接受分割剂量分别为2、4、6、8、10及12 Gy的射线照射,总照射剂量24～42 Gy。流式细胞术检测各照射组及对照组细胞的ABCG2、CD44及CD133表达率;各组细胞接种裸鼠皮下,检测其体内成瘤能力。【结果】各照射组细胞的ABCG2表达率均明显上调,以Hela-R2组(4 Gy×10次)细胞的表达率最高[(46.89±1.20)%],且随着照射次数的增加而升高(P<0.05);各组细胞的CD44及CD133表达率变化均无明显趋势;Hela-R2组细胞的裸鼠体内成瘤能力最高,其次为Hela-R5组(10 Gy×3次)。【结论】分割剂量射线照射可在体外富集宫颈癌肿瘤干细胞,以分割剂量为4 Gy组富集效果最好,诱导Hela细胞株表达ABCG2显著上调,及提高其裸鼠体内成瘤能力。[Objective] The aim of the study was to investigate the fractionated-dose irradiated Hela cells on the expression of cancer stem cell related proteins and the ability of in-vivo tumorigenicity, and to explore the feasibility of enriching cervical cancer stem cells by this method. [Methods] Hela cells were treated with different fractionated rays of dose 2, 4, 6, 8, 10, and 12 Gy, cumulatively 24-42 Gy. The expressing rates of ABCG2, CD44, and CD133 in irradiation groups and control group were detected by flow cytometry. Cells in each group were inoculated subcutaneously into nude mice to test their tumorigenicity in vivo. [ Results ] The expression of ABCG2 was significantly increased in irradiation groups,which was the highest in Hela-R2 group [ （46.89 ＋- 1.20）% ] and much more increased with more frequencies of radiation （P 〈 0.05 ）. There was no significant trend in the expression of CD44 and CD133 among each group. Cells in Hela-R2 group （4 Gy x 10） exhibited strongest tumorigenicity in vivo, followed by Hela-R5 group （10 Gy x 3 ）. [ Conclusion ] Fractionated-dose irradiation may enrich cervical cancer stem cells in vitro, with the best effect in dose 4 Gy group. Increased expression of ABCG2 and tumorigenicity in nude mice could be induced in Hela cells by this way.

关 键 词：HELA细胞 分割剂量 放射治疗 肿瘤干细胞 

分 类 号：R737.33[医药卫生—肿瘤]