Stat3、HIF-1α、MMP-7和VEGF在肾母细胞瘤中的表达  

Stat3, HIF-1α, MMP-7 and VEGF in Wilms tumor expression and clinical significance

作　　者：宗国兰[1] 杜春林[2] 刘秉成[1] 刘小伶[2] 钟小梅[2] 魏艳红[2] 张歆萍[2] 关艳[2] 

机构地区：[1]承德医学院附属医院小儿外科,河北承德067000 [2]承德市中心医院

出　　处：《中国误诊学杂志》2012年第18期4881-4883,共3页Chinese Journal of Misdiagnostics

基　　金：河北省承德市2009年科学技术研究与发展计划项目（200922112）

摘　　要：目的研究血管内皮生长因子（vascularendothelialgrowthfactor，VEGF），信号传导与转录激活因子3（sig—naltransduccerandactivatoroftranscription3，Stat3）、低氧诱导因子HIF-1α（hypoxia-induciblefactor-1α，HIF-1α）和基质金属蛋白酶-7（MMP-7）在肾母细胞瘤（nephrohlastoma，Wilmgtumor，WT）中的表达及其及其与临床分期和病理分级间的关系。方法采用免疫组化SP法检测43例手术切除WT标本和24例小儿非肿瘤肾组织标本中Star3、HIF-1α、MMP-7、与VEGF的表达。结果24例对照组织标本中MMP-7未见表达，43例WT组织标本中MMP-7在胞浆表达率54．18±10．72（MVD值）。MMP-7在预后良好（favorablehistologicaltypes，FH）组中的阳性表达率明显低于在预后不良（unfavorablehistologicaltypes，UH）组中的阳性表达率（P〈0．05）；VEGF、Stat3及HIF-1α在WT组织中表达强度较正常肾组织显著增强（P〈0．05）。另外，临床分期Ⅲ～Ⅳ和预后不良病理类型的WT中Stat3及VEGF表达强度明显高于临床分期Ⅰ～Ⅱ的WT，预后不良病理类型及直径≥6am的WT中HIF-1α表达强度较预后良好型及直径〈6cm的WT升高。VEGF的表达与性别、年龄和病理学分型无关（P〉0．05），而与临床分期明显相关（P〈0．05）。结论WT组织中Stat3、HIF-1α、MMP-7和VEGF的表达与肿瘤侵袭转移和发展有关，可被认为是肾母细胞侵袭性标志，对WT对于靶向治疗肾母细胞瘤具有一定意义。Objective Detection of Wilms tumor （nephrohlastoma, wilms tumor, WT） tissues of Star3, HIF-1α, MMP-7and VEGF expression and clinical stage and pathological grade relationships. Methods Immunohistochemical SP method detected 43 cases of surgical specimens and 24 cases of children with WT non-tumor kidney tissues Star3, HIF-1α, MMP-7and VEGF expression. Results 24 cases in control tissues did ilot express MMP-7, 43 cases of WT tissue samples MMP-7 expression in the cytoplasm of 54.18± 10.72 （MVD）. MMP-7 in a good prognosis （favorable histological types, FH） positive expression group was significantly lower than in the poor prognosis （unfavorable histological types, UH） group, positive expression rate （P〈0. 001 ） ; The expression of Star3, H IF-1α and VEGF were significantly up-regulated in Wilms＇tumor tissues compared to those in adjacent tissues and normal kidney tis- sues （P〈0.05）. Stat3 and VEGF proteins in Wilms＇tumor tissues of Stage Ⅲ - Ⅳ were significantly higher than those of stage Ⅰ - Ⅱ The higher expression of HIF-1 in Wilms＇tumor tissues was shown in tumors with high risk histopathology . VEGF in Wilms tumor tissues was significantly higher than children of non-tumor kidney tissue （P 〈0. 05 ）. VEGF expression significantly correlated with the clinical stage （ P〈0.05 ）. Conclusions WT tissue Star3, HIF-1,MMP7 and VEGF expression and tumor invasion, metastasis and development, can be considered invasive signs of Wilms on WT＇s clinical stage and pathologic grade and prognosis significance.

关 键 词：Wilms瘤/代谢 STAT3转录因子/代谢 缺氧诱导因子1 α亚基/代谢 基质金属蛋白酶7/代谢 血管内皮生长因子A/代谢 

分 类 号：R737.11[医药卫生—肿瘤]