纤维素酶法提取玫瑰花渣多糖工艺研究  

Research on cellulase degradation extraction of polysaccharide in rose dregs

作　　者：张曰辉[1] 马耀宏[2] 史建国[2] 杨俊慧[2] 

机构地区：[1]山东轻工业学院,山东济南250353 [2]山东省科学院生物研究所,山东省生物传感器重点实验室,山东济南250014

出　　处：《山东科学》2012年第3期71-74,共4页Shandong Science

摘　　要：采用单因素实验和L9(34)正交试验,研究纤维素酶酶解时间、酶解温度、酶解pH、酶加量对多糖得率的影响。利用还原糖测定仪测定经酸水解的多糖。结果表明,酶的反应温度和酶解pH值是提取玫瑰多糖的主要因素。最佳工艺方案为:酶反应温度50℃,酶反应pH值为4.6,酶反应时间150 min,酶加量为4.5%。酶反应完后以料液比1∶30在100℃下提取5 h,在此工艺条件下,提取液中玫瑰多糖的得率为11.1%,可溶性多糖提取率为4.49%。We employ single factor test and orthogonal test design method L9 （34） to analyze such influence factors as enzymolysis temperature, enzymolysis pH, enzymolysis time and cellulose amount on the yield rate of the polysaccharide. We also use reducing sugar analyzer to determine the amount of polysaccharide. Results show that temperature and pH are two dominant factors. The optimal enzymolysis condition is temperature of 50 ℃, pH of 4.6, 150 min and cellulose amount of 4.5%. The extract ratio of rose dregs polysaccharide is 11.1% and the yield rate of soluble polysaccharide is 4.49% in condition of the ratio of rose dreas and water of 1： 30. temoerature of 100 ℃ and extract time of 5 h.

关 键 词：玫瑰花渣 多糖 纤维素酶 

分 类 号：R284.2[医药卫生—中药学]