膀胱癌患者尿沉渣中微卫星不稳定性的临床意义  

Microsatellite instability in urine sediments from patients with transitional cell carcinoma of bladder and its clinical value

作　　者：吕英谦 唐东起[1] 俞莉章[1] 丁义[1] 刘漓波[1] 郭应禄[1] 

机构地区：[1]北京医科大学第一医院泌尿外科,100034

出　　处：《中华外科杂志》2000年第4期294-296,共3页Chinese Journal of Surgery

基　　金：卫生部基金资助项目!(981256)

摘　　要：目的　探讨微卫星不稳定性 (MSI)在膀胱癌中的表现及其临床诊断价值。　方法　利用PCR方法检测了 35例膀胱癌患者术前尿沉渣中 13个微卫星位点 ,并分析了微卫星不稳定性 (MSI)及杂合性丢失 (LOH)。对其中 2 5例患者进行了随访。　结果　 35例膀胱癌经尿沉渣微卫星分析 ,31例 (88 6 % )可检出膀胱癌。 12例复发肿瘤中 10例可见微卫星改变 (83% ) ,3例首先发现尿沉渣微卫星改变 ,经 1～ 6个月继续随访后 ,经膀胱镜证实发现膀胱肿瘤。　结论　将微卫星DNA作为分子标记应用于膀胱癌早期诊断及监测复发 。Objective To investigate the expression of microsatellite instability in transitional cell carcinoma of bladder and to detect its diagnostic value. Methods Urine samples from 35 patients with transitional cell carcinoma of bladder (TCC) were analyzed by PCR method. 25 patients were followed up to detect microsatellite instability in their urine sediments.Results Microsatellite changes (including MSI and LOH) were detected in 88 6% of urine sediments(31 of 35 patients). Microsatellite changes were detected in urine sediments of 10 of 12 patients with tumor recurrence, in which the existence of tumor cells in the urine of 3 patients had been correctly predicted before cytoscopic evidence from 3 to 6 months. Conclusion Microsatellite analysis of urine sediment may be a novel and potentially clinical tool for the diagnosis and follow up of bladder cancer patients.

关 键 词：膀胱肿瘤 DNA 尿沉渣 微卫星不稳定性 

分 类 号：R737.14[医药卫生—肿瘤]