人骨形态发生蛋白2基因修饰自体骨髓间充质干细胞移植促进兔下颌骨牵张成骨实验的组织形态学观察  

Transplantation of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells promotes bone regeneration in rabbit mandibular distraction osteogenesis: A histomorphological study

作　　者：黄旋平[1] 周诺[1] 江献芳[1] 宋继传[1] 李华[1] 谢庆条[1] 

机构地区：[1]广西医科大学附属口腔医院口腔颌面外科,广西壮族自治区南宁市530021

出　　处：《中国组织工程研究》2012年第23期4242-4246,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30760273;30960421);广西壮族自治区自然科学基金资助项目(桂科自0991013Z)~~

摘　　要：背景:如何提高牵张成骨过程中新骨形成的速度和质量,缩短牵张成骨治疗时间,减少并发症的发生是目前该领域的研究热点。目的:观察人骨形态发生蛋白2基因修饰自体骨髓间充质干细胞移植对兔下颌骨牵张成骨新骨形成的促进作用。方法:36只新西兰白兔随机摸球法分为3组。建立牵张成骨动物模型,在固定期第2天,实验组于牵张间隙注射人骨形态发生蛋白2基因修饰的自体骨髓间充质干细胞;对照组注射等量自体骨髓间充质干细胞;空白组注射等量生理盐水。结果与结论:在固定期2周及6周实验组牵张区骨小梁形成质量明显好于对照组和空白组。证实骨形态发生蛋白2基因修饰的自体骨髓间充质干细胞移植能有效促进兔下颌骨牵张成骨新骨形成。BACKGROUND： How to promote new bone formation and regeneration quality, shorten hospitalization and treatment times, and decrease related complications has recently been the edge-cutting focus in the field. OBJECTIVE： To investigate the effect of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells transplantation on promoting rabbit bone regeneration in mandibular distraction osteogenesis by histomorphological method. METHODS： Thirty-six New Zealand white rabbits were randomly divided into three groups with 12 rabbits in each. All the objects were prepared into distraction osteogenesis surgical models on the right mandible. On the 2~~ day of consolidation period, rats in the experimental group, control group and blank group were injected with the same amount of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells, autogenous bone marrow mesenchymal stem cells and physiological saline at distraction gap, respectively. RESULTS AND CONCLUSION： The quality of bone trabecula of the experimental group was better than that of the control group and blank group at 2 and 6 weeks of consolidation period. The transplantation of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells can effectively promote bone regeneration in rabbit mandibular distraction osteogenesis.

关 键 词：骨形态发生蛋白2 基因修饰 骨髓间充质干细胞 牵张成骨 组织形态学 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]