单核细胞趋化蛋白1与神经干细胞的体外迁移  

In vitro migration of monocyte chemoattractant protein 1 and neural stem cells

作　　者：董岳[1] 康波[2] 丁鹏[1] 戴孝森[1] 王崇谦[1] 王伟民[1] 尚亚军[1] 王进昆[1] 

机构地区：[1]昆明医学院第一附属医院神经外科,云南省昆明市650000 [2]昆明市延安医院神经外科,云南省昆明市650000

出　　处：《中国组织工程研究》2012年第23期4279-4283,共5页Chinese Journal of Tissue Engineering Research

基　　金：2008年云南应用基础研究面上项目(2008ZC132M;0920033):趋化因子及其受体在NSCs迁移中的作用的研究;MCP-1/CCR2通路在神经干细胞趋化迁移中作用的研究~~

摘　　要：背景:研究表明趋化因子基质细胞衍生因子1及血管内皮生长因子参与神经干细胞的迁移。目的:观察趋化因子单核细胞趋化蛋白1及其受体CCR2对神经干细胞体外迁移的作用。方法:分离培养胎鼠海马组织神经干细胞,体外传代扩增及鉴定;通过细胞免疫荧光及RT-PCR检测其受体CCR2表达情况;通过琼脂糖下细胞迁移实验观察50,100,200,300,500μg/L单核细胞趋化蛋白1对神经干细胞的体外趋化作用。结果与结论:细胞免疫荧光及RT-PCR检测证实胎鼠海马来源神经干细胞表达趋化因子受体CCR2;琼脂糖下细胞迁移实验显示,体外条件下单核细胞趋化蛋白1可趋化神经干细胞迁移,且趋化迁移作用随质量浓度增加而增强,抗CCR2多克隆抗体可对抗其趋化迁移作用。BACKGROUND： Studies have shown that the chemokine stromal cell derived factor-1 and vascular endothelial growth factor have the ability to make neural stem cells （NSCs） migration. OBJECTIVE： To explore the effect of monocyte chemoattractant protein 1 （MCP-1） and its receptoron the migrati on of NSCs in vitro. METHODS： Hippocampal NSCs from SD fetal rats were isolated and cultured; meanwhile they could be amplified and identified in vitro; The expression of CCR2 was detected with immunofluorescence and RT-PCR; Effect of MCP-1 with different concentrations （50, 100, 200, 300, 500 ng/mL） on NSCs in vitro migration was obsened under agarose. RESULTS AND CONCLUSION： Immunofluorescence and RT-PCR results showed that NSCs from fetal rat hippocampal could express chemokine receptor CCR2; and the migration experiment under agarose showed that the MCP-1 with different concentrations could induce the migration of NSCs in vitro and with the higher the dose the better the migration. But the anti-CCR42 polyclonal antibodies could inhibit the migration of NSCs.

关 键 词：单核细胞趋化蛋白1 神经干细胞 趋化因子 细胞迁移 CCR2 

分 类 号：R394.2[医药卫生—医学遗传学]