胃肠道富集Krüppel样因子表达与胃癌患者预后的关系  

Association between gut-enriched Kruiippel-like factor and prognosis of patients with gastriccancer

作　　者：李明学[1] 王权[1] 王宾[1] 严东旺[1] 唐华美[1] 彭志海[1] 于振海[1] 

机构地区：[1]山东大学附属千佛山医院普通外科,济南250014

出　　处：《中华胃肠外科杂志》2012年第7期732-735,共4页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的探讨KLF4在胃癌组织中的表达及其与胃癌患者预后的关系。方法收集上海交通大学附属第一人民医院2004＋2009年胃癌根治性手术标本264例．应用实时定量PCR和免疫组织化学染色方法检测KLF4在胃癌及癌旁组织中的表达。结果KLF4蛋白在胃癌和癌旁组织中的阳性表达率分别为43．2％（114／264）和79．2％（209／264），差异有统计学意义（P〈0．01）。KLF4表达与浸润深度（P〈0．05）、淋巴结转移（P〈0．01）、远处转移（P〈0．05）及临床分期（P〈0．05）有关。KLF4阳性与阴性表达患者5年总体生存率分别为65．2％和41．2％．5年无瘤生存率分别为50．4％和36．2％。差异均有统计学意义（P〈0．05）。KLF4表达是胃癌患者总体生存（HR=2．89，95％CI：1．18-9．23）和无瘤生存（HR=2．14，95％CI：1．03-4．37）的独立预后因素。结论KLF4在胃癌组织中的表达显著下调：KLF4有可能成为预测胃癌患者预后的生物学标志物。Objective To explore the expression of gut-enriched Kriippel-like factor 4 （KLF4） in gastric cancer, and its association with prognosis. Methods Surgical specimens were collected from 264 patients undergoing radical surgery between 2004 and 2009 in the Affiliated Qianfoshan Hospital, Shandong University. KLF4 mRNA level of specimens was detected by real-time PCR. KLF4 protein expression was measured by immunohistochemistry on tissue microarray, which contained primary gastric cancer, corresponding para-cancerous tissue, and paired lymph node metastases. Results Real-time PCR revealed that mRNA level of KLF4 was down-regulated in gastric cancer compared with paired normal gastric mucosa. Immunohistochemistry on tissue microarray showed gastric cancer tissues had significantly lower KLF4 levels compared with paired normal gastric tissues. By univariate and multivariate analysis, KLF4 was a significant predictor of survival and recurrence. Conclusion KLF4 expression is significantly down-regulated in gastric cancer, and is an independent predictor of survival and recurrence.

关 键 词：胃肿瘤 胃肠富集Kriippel样因子4 预后 

分 类 号：R735.2[医药卫生—肿瘤]