定量测定储粮害虫胃毒作用的方法——混药薄片法  

A method to quantitative determine the stomach poison on stored grain pests—mixed drug sheet method

作　　者：曹阳[1,2] 刘芳[2] 李燕羽[1] 张涛[1] 

机构地区：[1]国家粮食局科学研究院,北京100037 [2]河南工业大学粮油食品学院,郑州450052

出　　处：《应用昆虫学报》2012年第4期1072-1077,共6页Chinese Journal of Applied Entomology

基　　金："十一五"国家科技支撑计课题"储备粮减损生物测防新技术和仪器研发"的内容"防治储粮害虫高效苏云金芽孢杆菌生物杀虫剂研究开发"(2009BADA0B005-3)

摘　　要：本实验制定一种定量测定储粮害虫胃毒作用的方法,命名为混药薄片法。通过试验验证饲料薄片对烟草甲Lasioderma serricorne(Fabricius)的幼虫成活率、发育历期、虫体重量、产卵量等生长发育无影响。在排除烯虫酯对烟草甲的触杀作用的前提下,将混有烯虫酯溶液的混药薄片作为饲料饲养烟草甲3龄幼虫,得到烯虫酯对烟草甲3龄幼虫的准确致死剂量为(0.46±0.03)μg。A new method to quantitatively determine the lethal dose of poison ingested by stored grain pests, the mixed drug sheet method, is described. Experiments verified that the method had no effect on Lasioderma serricorne＇ s survival rate, developmental period, body weight, fecundity, etc. By feeding 3th instar larvae of L. serricorne with a feedstuff sheet mixed with solution of methoprene, we excluded the contact toxicity of methoprene and obtained the exact lethal dose （0. 46 ± 0. 03 ） ug/each larvae.

关 键 词：胃毒剂 混药薄片 烟草甲 烯虫酯 

分 类 号：S379.5[农业科学—农产品加工]