NBS1在大鼠唾液腺放射损伤中的表达变化及意义  

Expression of NBS1 in the salivary gland of radiation-injured rats

作　　者：林丹[1] 王代友[1] 杨亦萍[1] 卿海云[1] 曹阳[1] 陈超梅[1] 沈洁[1] 欧健波[1] 

机构地区：[1]广西医科大学附属口腔医院颌面外科,南宁530021

出　　处：《中华放射医学与防护杂志》2012年第3期241-244,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（30960420）;广西医疗卫生重点科研课题（桂卫重2010082）

摘　　要：目的探讨大鼠γ射线照射后唾液腺组织NBS1基因和蛋白的表达，及其在唾液腺上皮细胞放射性损伤修复中的调控作用。方法将80只大鼠按随机数字表法均分为健康对照组和照射组，各40只。照射组大鼠以 ^60Coγ 射线分次照射，每次3Gy，隔天1次，共5次，累积剂量为3、6、9、12和15Gy。应用电镜观察唾液腺细胞超微结构变化。用免疫组织化学技术（IHC）和反转录聚合酶链技术（RT-PCR）分别检测照射后2-4h的腮腺、颌下腺NBS1mRNA和蛋白的表达情况。结果照射组腮腺组织萎缩和损伤程度重于颌下腺。腮腺组织吸收剂量为9．12和15Gy时、颌下腺组织吸收剂量为9和12Gy时，NBS1mRNA表达水平明显降低（t=7．10、17．93、20．86，P〈0．05；t=3．13和7．53，P〈0．05）。照射组腮腺浆液细胞吸收剂量为9．12和15Gy时、导管上皮细胞剂量为12和15Gy时，差异有统计学意义（t=4．29、17．91、91．29，P〈0．05；t=3．09和5．62，P〈0．05）。颌下腺浆液细胞在12Gy时，NBS1蛋白表达明显减少（t=4．61和11．84，P〈0．05）。颌下腺导管上皮细胞及黏液细胞其NBS1蛋白表达在整个照射过程中未见明显变化。结论 γ射线照射后大鼠唾液腺组织NBS1mRNA和蛋白水平均出现下降，推测NBS1可能参与唾液腺放射损伤和修复的过程。Objective To investigate the expressions of NBS1 mRNA and protein in the salivary gland of irradiated rats and explore the role of NBS1 in the repair of radiation injury of salivary gland epithelial cells. Methods Eighty rats were randomly divided into two groups for radiation and control （n =40 each）. The rats were fractionally exposed to 3 Gy of ^60Co γ-rays once in two days, leading to an accumulation dose of 3,6,9,12,15 Gy. The sham-irradiated controls were anesthetized in parallel but without irradiation. After 2-4 h of irradiation, the rats were sacrificed, IHC and RT-PCR were used to detect the expressions of NBS1 protein and mRNA in parotid and submandibular glands, and the ultra- structural changes in the glands were observed by a transmission electron microscopy. Results After irradiation, the salivary glands became atrophy and the parotid gland cells were damaged more serious than the submandibular gland cells. Compared with the controls, with the groups of dose, at 9,12,15 Gy in parotid gland （t =7.10, 17.93, 20. 86, P 〈0. 05） , at 12, 15 Gy in the submandibular gland （t =3.13, 7.53,P 〈0. 05） ,the expression of NBS1 mRNA was reduced. With the groups of dose at 9, 12, 15 Gy in parotid gland （t=4.29,17.91,91.29,P〈0.05）, the dose at 12, 15 Gy in submandibular gland （t = 4. 61,11.84,P 〈 0. 05）, the expression of NBS1 protein in serous cells, and the dose at 12, 15 Gy in parotid gland ductal epithelial cell （t = 3.09, 5.62, P 〈 0. 05） were reduced. But in the ductal epithelial cells as well as muoass cells in the submandibualr gland were steadily. Conclusions After irradiation, NBS1 at both protein and mRNA levels was dropped in the salivary gland of rats, which might contribute to the repair of radiation injury of salivary gland.

关 键 词：NBS1 大鼠 唾液腺 上皮细胞 放射损伤 

分 类 号：R739.91[医药卫生—肿瘤]