子宫内膜癌SREBP1表达及SREBP1 shRNA对内膜癌细胞生物学行为的影响  

SREBP1 expression in endometrial cancer and the influence of SREBP1 shRNA on the biological behavior of endometrial cancer cells

作　　者：李卫华[1,2] 周洁[3] 顾伟亭[1,2] 姜洁[1] 孔北华[1] 

机构地区：[1]山东大学齐鲁医院妇产科,济南250012 [2]托马斯杰斐逊大学Kimmel癌症中心癌症生物学,费城19107 [3]第四军医大学西京医院内分泌代谢科,西安710032

出　　处：《现代妇产科进展》2012年第6期458-461,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(No:81072121;81173614);山东省自然科学基金项目(No:Z2008C13);山东省科学和技术发展规划基金项目(No:2011GSF12122)

摘　　要：目的:探讨胆固醇调节元件结合蛋白质1(SREBP1)在子宫内膜癌中的表达情况及SREBP1 shRNA对子宫内膜癌细胞AN3-CA生物学行为的影响。方法:采用免疫组织化学染色、实时定量逆转录聚合酶链反应、蛋白免疫印迹、Transwell体外细胞侵袭试验等方法分析SREBP1在子宫内膜组织中的表达及其对细胞生物学行为的影响。结果:SREBP1在正常子宫内膜组织中低表达,在子宫内膜癌组织中高表达,且免疫组织化学染色H-Score评分在子宫内膜癌高分化组和中、低分化组之间具有统计学差异(P<0.001);SREBP1 shRNA可减慢子宫内膜癌细胞AN3-CA的增殖速度并降低AN3-CA细胞的侵袭能力,均具有统计学差异(P<0.001)。结论:SREBP1的表达与活化参与子宫内膜癌的进展;SREBP1 shRNA可降低子宫内膜癌细胞的增殖和侵袭能力,表明内源性SREBP1参与子宫内膜癌的进展与转移,为子宫内膜癌潜在的分子靶向治疗提供了一定的理论基础。Objective：To detect sterol regulatory element binding protein1（SREBP1） expression in endometrial cancer and to explore the influence of SREBP1 shRNA on the biological behavior of endometrial cancer cells.Methods：Immunohistochemistry staining,quantitative real-time reverse transcription-polymerase chain reaction,Western blot,Transwell invasion assay were conducted to detect SREBP1 expression and to analyze the influence of SREBP1 shRNA on the biological behavior of endometrial cancer cells.Results：SREBP1 was expressed in normal endometrium and overexpressed in endometrial cancer;IHC H-Score ratings between highly differentiated endometrial cancer group and moderately to low differentiated group exhibited a significant difference（P0.001）.SREBP1 shRNA decreased cell proliferation and invasion capacity of AN3-CA cells（P0.001）.Conclusions：There was a close correlation between SREBP1 overexpression,over-activation and progression of endometrial cancer.SREBP1 shRNA reduces cell proliferation and invasion capacity,indicating that endogenous SREBP1 plays a role in development and metastasis of endometrial cancer.Furthermore,the results of this study indicate that SREBP1 could serve as a potential molecular target in the treatment of endometrial cancer.

关 键 词：子宫内膜肿瘤 胆固醇调节元件结合蛋白1 短发夹RNA 细胞侵袭力 

分 类 号：R737.33[医药卫生—肿瘤]