骨髓源神经干细胞移植治疗中枢神经系统损伤的蛋白质组学研究  

Proteomic analysis of autologous bone marrow derived neural stem cell transplantation for treatment of central nervous system injury

作　　者：马灿[1] 戴宜武[1] 徐如祥[1] 

机构地区：[1]安徽医科大学北京军区总医院临床学院.北京军区总医院附属八一脑科医院,北京100700

出　　处：《中华神经医学杂志》2012年第7期691-694,共4页Chinese Journal of Neuromedicine

摘　　要：目的利用同位素标记相对和绝对定量（iTRAQ）技术以及串联质谱技术分析中枢神经系统损伤患者干细胞移植前术后脑脊液蛋白质表达变化情况。方法收集正常成人以及中枢神经系统损伤患者移植前后脑脊液，组内等量混合后利用高效液相色谱柱去除高丰度蛋白，样本经iTRAQ试剂标记后利用二维色谱分离，经串联质谱鉴定并相对定量。结果质谱得到的多肽片段经谱库搜索鉴定的蛋白共59个，其中患者移植前脑脊液与正常对照组脑脊液存在差异的蛋白质有13个．5个呈高表达，8个呈低表达。患者移植后脑脊液有7种蛋白质与正常对照组表达无差异。结论蛋白质组学研究方法可以用来寻找神经系统损伤标志物，为神经系统损伤以及修复的机制研究提供基础。Objective To apply the isobaric tagging for relative and absolute quantitation （iTRAQ） and liquid chromatography-tandem mass spectrometry （LC-MS/MS） to identify the proteins differentially regulated in CSF of patients before and after stem cell transplantation. Methods CSF samples from patients with central nervous system injury before and after stem cell transplantation and fi＇om normal controls were collected; selective removal of high-abundant proteins was performed by high performance liquid chromatography; proteins were differentially identified by iTRAQ-coupled LC-MS/MS. Results Totally, 59 proteins were identified. As compared with control group, the patients before transplantation had 5 over-expressed proteins and 8 under-expressed proteins; only 7 proteins in patients after transplantation showed no significantly different expression as compared with those in control group. Conclusion Protein expression is significantly changed in CSF of patients with central nervous system injury after neural stem cell transplantation, suggesting that the proteomics research can help us to find markers of nervous system damage and is important to mechanism study on nervous system damage and repair.

关 键 词：中枢神经系统损伤 蛋白质组学 干细胞移植 脑脊液 

分 类 号：R741[医药卫生—神经病学与精神病学]