TESTIN基因、RUNX3基因在结直肠癌组织中的表达及临床意义  

Expression of TESTIN and RUNX3 in Colorectal Carcinoma Tissue and Its Clinical Significance

作　　者：龙结根[1] 张志坚[2] 张才全[3] 

机构地区：[1]重庆市巴南区人民医院普外科,重庆401320 [2]重庆市巴南区人民医院ICU,重庆401320 [3]重庆医科大学附属第一医院胃肠外科,重庆400016

出　　处：《南昌大学学报（医学版）》2012年第4期53-57,共5页Journal of Nanchang University：Medical Sciences

摘　　要：目的检测人结直肠癌组织中侯选抑癌基因TESTIN及RUNX3的表达,探讨其与人结直肠癌临床病理特征的关系。方法应用免疫组化SP法检测60例结直肠癌组织及其相应的癌旁正常组织中TESTIN基因、RUNX3基因的表达。结果 60例结直肠癌组织中TESTIN基因阳性(31.7%)、强阳性(5.0%)表达率显著低于其相应的癌旁正常组织(86.7%、60.0%),差异均有统计学意义(均P<0.01)。60例结直肠癌组织中RUNX3基因阳性(26.7%)、强阳性(1.7%)表达率显著低于其相应的癌旁正常组织(81.7%、55.0%),差异均有统计学意义(均P<0.01)。60例结直肠癌组织中,TESTIN基因、RUNX3基因表达与患者的性别、年龄、部位均无相关性(r=-0.452、-0.245、-0.331,均P>0.05),与T分期(T3期+T4期)、Dukes分期(C期+D期)、有淋巴结转移、有远处转移、分化程度(低分化)均呈正相关(r=2.352、1.485、1.867、2.541、0.885、3.214,均P<0.05)。TES-TIN基因表达与RUNX3基因表达呈正相关(r=6.587,P<0.05)。结论 TESTIN基因、RUNX3基因与结直肠癌的生物学行为密切相关,可能作为判断结直肠癌患者预后及浸润转移的生物学指标,也可能成为结直肠癌靶向治疗的一个新靶点。Objective To detect the expression of candidate tumor suppressor gene TESTIN and RUNX3 in human colorectal carcinoma,and to explore its relationship to clinical and pathological features of colorectal carcinoma.Methods The expression of TESTIN and RUNX3 was detected by immunohistochemistry in 60 colorectal carcinoma specimens and their corresponding adjacent normal tissues.Results The rates of positive expression and strong positive expression of TESTIN in colorectal cancer tissues were significantly lower than those in adjacent normal tissues（31.7% and 5.0% vs 86.7% and 60.0%,respectively;P0.01）.In addition,the rates of positive expression and strong positive expression of RUNX3 in colorectal cancer tissues were significantly lower than those in adjacent normal tissues（26.7% and 1.7% vs 81.7%and 55.0%,respectively;P0.01）.The expression of TESTIN and RUNX3 was positive correlated with infiltration,poor differentiation,Dukes stage（C and D）,T stage（T3 and T4）,lymph node metastasis and distant metastasis（r=2.352,1.485,1.867,2.541,0.885 and 3.214,respectively;P0.05）,but not with gender,age and carcinoma location（r=-0.452,-0.245 and -0.331,respectively;P0.05）.Furthermore,there was a positive correlation between TESTIN expression and RUNX3expression（r=6.587,P0.05）.Conclusion Both TESTIN and RUNX3are closely related to biological behaviors of colorectal carcinoma,and may be valuable as markers for assessing the prognosis and metastasis of colorectal carcinoma and as new targets for treating colorectal carcinoma.

关 键 词：结直肠癌 癌旁正常组织 Testin基因 RUNX3基因 免疫组化 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]