米非司酮对宫颈癌Hela细胞的增殖抑制作用  

Effects of Mifepristone on cell proliferation of human cervical carcinoma cell lines Hela in vitro

作　　者：孙丽君[1] 郑英[1] 崔金全[1] 王伟娟[1] 黄壮士[1] 谭丽[1] 

机构地区：[1]河南医科大学第二附属医院妇产科,郑州450003

出　　处：《河南医科大学学报》2000年第3期258-259,共2页Journal of Henan Medical University

基　　金：河南省教委自然科学研究基金资助项目!973 2 0 0 13

摘　　要：目的 :探讨米非司酮对人子宫颈癌Hela细胞增殖的影响。方法 :采用MTT测定 ,观察不同浓度米非司酮 (10 ,5 ,2 .5 μmol/L)对Hela细胞增殖的抑制作用 ;通过ABC免疫组化法 ,检测用药后Hela细胞Ki 6 7抗原表达的变化。结果 :3个浓度的米非司酮对Hela细胞增殖均有抑制作用 ,并呈剂量依赖性 ,其中以 10 μmol/L浓度抑制作用最强 ,抑制率达 5 7.6 9% ;2 .5 μmol/L米非司酮可使Ki 6 7抗原表达明显减少。 结论 :米非司酮体外对PR阳性的人宫颈癌细胞有明显的抑制作用 ,通过降低细胞Ki 6 7抗原的表达而抑制其增殖。Aim:To investigate the effects of Mifepristone on cell proliferation of human cervical carcinoma cell lines Hela in vitro.Methods:The antiproliferation effects of three different concentrations of Mifepristone (10,5,2.5 μmol/L) on Hela cell lines were detected with MTT assay.Immunohistochemical technique ABC were used to determine the changes of Ki 67 antigen expression after treatment with Mifepristone.Results:Three different concentrations of Mifepriston dose dependently inhibited the proliferation of Hela cells.The inhibition by Mifepristone plateaued at 10 μmol/L(57.68% inhibition).Mifepristone at 2.5 μmol/L decreased the expression of Ki 67 antigen of Hela cells.Conclusion:In vitro,Mifepristone significantly inhibited the growth and the proliferation of human cervical carcinoma cell lines that are progestin receptor positive and reduced the expression of Ki 67 antigen of the cells.

关 键 词：米非司酮 HELA细胞 KI-67抗原 子宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤] R984[医药卫生—临床医学]