RTPCR技术在胃肠癌外周血微转移检测中的应用  

Application of RT PCR technique in assay of micrometastases in gastro intestinal carcinoma

作　　者：刘伟[1] 张亚历[2] 

机构地区：[1]解放军254医院,天津300142 [2]第一军医大学南方医院消化研究所,广东广州510515

出　　处：《中国癌症杂志》2000年第2期175-177,共3页China Oncology

摘　　要：胃肠肿瘤是常见的恶性肿瘤 ,而且大部分较早地通过血道和淋巴管发生微转移 ,术后复发率较高。因而早期诊断和及时发现外周血微转移现象对于临床治疗和判断预后有着重要意义。本文综述近年来利用分子生物学技术尤其是具有较高敏感性和特异性的逆转录聚合链反应 (RT PCR ,reversetranscription polymerasechainreac tion)技术 ,检测肿瘤组织相关分子 ,如CK19,CK2 0 ,CEA和CD44v6表达的mRNA 。Gastro intestinal carcinoma is acommon malignancy, Because the tumour cells can enter into blood vessels and lymphatic system early and often recur after surgicalresection, early diagnosis and discovery of micrometastases is important in therapy and judgement of prognosis. This thesis summarizes some successes in the field of molecular techniques especially RT PCR technique (reverse transcription polymerase chain reaction) which has developped rapidly in recent years. RT PCR technique in sensitive and specific in analysis and estimation of tumor micrometastases by detecting the molecules interraleted with tumors. These molecules include mRNA of CK19, CK20, CEA, CD44 and some apoptosis genes.

关 键 词：RT-PCR 胃肠肿瘤 外周血微转移 

分 类 号：R735.04[医药卫生—肿瘤] R730.43[医药卫生—临床医学]