乳腺癌中14-3-3σ的表达及其与Bcl-2和Bax的相关性研究  

Detection the relationship between expression of 14-3-3σ and Bcl-2/Bax in breast cancer

作　　者：杨桦[1] 马洪波[1] 王子兵[1] 崔树德[1] 

机构地区：[1]河南省肿瘤医院乳腺病诊疗中心,郑州450003

出　　处：《中国肿瘤临床与康复》2012年第3期224-227,共4页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：国家自然科学基金青年基金(81000914)

摘　　要：目的探讨乳腺癌中及其与Bcl-2和Bax表达的关系,进一步研究乳腺癌中14-3-3σ的表达情况及与14-3-3σ相关的细胞凋亡调控的分子机制。方法应用免疫组织化学方法检测58例乳腺癌组织中14-3-3σ,Bcl-2和Bax的表达情况。对58例乳腺癌组织中14-3-3σ的表达水平与相应临床病理参数进行统计分析,并根据随访资料对患者进行生存分析。应用Spearman相关分析分别统计14-3-3σ表达与Bcl-2和Bax蛋白表达的相关关系。结果 14-3-3σ在乳腺癌组织中阳性表达率为70.7%(41/58),Bcl-2的阳性表达率为62.1%(36/58),Bax的阳性表达率为32.8%(19/58)。14-3-3σ蛋白表达与肿瘤大小、腋窝淋巴结的转移和TNM分期有关。随访的58例乳腺癌病例中,14-3-3σ阳性组的5年累积生存率为51.2%(21/41),14-3-3σ阴性组的5年累积生存率为82.4%(14/17),14-3-3σ阳性组的5年累积生存率明显低于14-3-3σ阴性组,两者差异有统计学意义(P<0.05)。相关性分析结果表明,14-3-3σ蛋白的表达与Bcl-2蛋白的表达呈正相关,而与Bax蛋白的表达呈负相关(P<0.05)。结论 14-3-3σ参与了乳腺癌的发生和发展,并可作为衡量患者预后的新指标。14-3-3σ可能是通过调节凋亡相关蛋白Bcl-2和Bax的表达,从而参与了抑制肿瘤细胞凋亡的过程。Objective Detection the relationship between expression of 14-3-3σand Bcl - 2/Bax in breast cancer and investigation the molecular mechanism of cell apoptosis related with 14-3-3σ. Methods Explore the expression of 14-3-3σ, Bcl - 2 and Bax in breast cancer by using immunohistochemistry. Investigation the relationship between expression of 14-3-3σ and clinico- pathological factors. Then we analysed the prognostic of patients. Study the relationship between 14-3-3σ and Bcl - 2/Bax by Spearman correlation analysis. Results The positive rates of 14-3-3σ, Bcl - 2 and Bax in breast cancer was 70.7% （41/58） , 62.1% （36/58） and 32.8% （19/58） respec- tively. 14-3-3σexpression had a close correlation with tumor size, lymph node of axillary and TNM. 5 -years survival rate of14-3-3σ rpositive and negative group was 51.2% and 82.4% respectively. The negative group of 14-3-3σ was better than positive group. The expression of 14-3-3σ had positive relation with Bcl -2 and negative relation with Bax. Conclusions 14-3-3σ participate in the progression of breast cancer and could be a new prognostic factor. 14-3-3σ maybe participate in inhibition the cell apoptosis by modulating Bcl- 2/Bax balance.

关 键 词：乳腺癌 14-3-3σ 凋亡 预后 

分 类 号：R737.9[医药卫生—肿瘤]