Bc1-2高表达小鼠模型的建立  

Establishment of mouse model highly expressing Bcl-2 protein

作　　者：张燕飞[1] 降建新[1,2] 王瑛[1] 张夔鸣[1] 范慧敏[2] 孙志扬[1] 

机构地区：[1]同济大学附属东方医院神经外科,上海200120 [2]同济大学附属东方医院胸外科,上海200120

出　　处：《同济大学学报（医学版）》2012年第3期1-4,共4页Journal of Tongji University(Medical Science)

基　　金：上海市浦东新区社发局科研基金(PW08-07)

摘　　要：目的建立Bcl-2高表达的转基因小鼠品系,为在活体动物上研究bcl-2蛋白的功能提供动物模型。方法构建含人Bcl-2基因的质粒;通过显微注射将该质粒转入C57BL/6J×CBA的杂交小鼠的受精卵,并植入假孕母鼠的子宫,PCR检测后携带人Bcl-2基因的小鼠即为"Founder"鼠;将"Founder"鼠与C57BL/6J×CBA杂交小鼠不断回交,经PCR和Western bilotting筛选后即可获得阳性小鼠。结果共获得5只"Founder"鼠,其中#6、#14成功建系,连续回交繁殖7代后,PCR和Western筛选后获得bcl-2高表达阳性小鼠42只。结论成功将人bcl-2基因转入小鼠基因组内,并稳定传代,建立Bcl-2高表达小鼠,为进一步研究Bcl-2蛋白提供了很好的动物模型。Objective To establish a mouse model highly expressing Bcl-2 protein for study of Bcl-2 function in vivo. Methods The plasmid containing human bcl-2 gene was constructed, which was transferred to fertilized eggs of C57BL/6J× CBA hybrid mice by microinjection; the fertilized eggs were implanted in the uterus of falsely pregnant female mice. The bcl-2 gene-carrying mice were the ＂Founder＂ mice, the ＂Founder＂ mice and C57BL/6J × CBA hybrid mice were continuously backcrossed, and the transgenic mice expressing Bcl-2 were obtained by screening with PCR and Western blotting. Results Five ＂Founder＂ mice were obtained; the strains were successfully established from #6 and #14 mice. After 7 generations of backcross breeding and screening by PCR and Western blot, 42 transgenic mice highly expressing bcl-2PCR were obtained. Conclusion The mouse model highly expressing Bcl-2 protein has been successfully established in this study.

关 键 词：BCL-2蛋白 转基因 高表达 小鼠 

分 类 号：R394.8[医药卫生—医学遗传学]