枸橼酸铁铵对小胶质细胞释放炎性因子的作用  

THE EFFECT OF FERRIC AMMONIUM CRITRATE ON MICRGLIA RELEASING PRO-INFLAMMATION CYTOKINES

作　　者：王佳[1] 宋宁[1] 姜宏[1] 王俊[1] 谢俊霞[1] 

机构地区：[1]青岛大学国家生理学重点(培育)学科,山东省神经相关疾病的机制与防治重点实验室,山东青岛266071

出　　处：《青岛大学医学院学报》2012年第3期189-190,194,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：科技部国家重点基础研究发展计划973计划子课题(2011CB504102);山东省自然科学基金(ZR2010HM003);山东省医药卫生科技发展计划项目(2009QZ005)

摘　　要：目的观察枸橼酸铁铵(FAC)对小胶质细胞释放炎性因子的作用。方法分别用脂多糖(LPS)、FAC、LPS+FAC刺激小胶质细胞,采用酶联免疫吸附试验方法检测培养基中白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的含量。结果对照组和FAC组几乎检测不到炎性因子的释放,LPS组IL-1β、TNF-α释放量较对照组显著增加(F=38.864、102.504,q=8.204、14.369,P<0.01),LPS+FAC组IL-1β、TNF-α释放量较LPS组显著增加(q=4.517、5.610,P<0.01)。结论 FAC可以刺激激活状态小胶质细胞释放大量炎性因子。Objective To investigate the effect of ferric ammonium citrate （FAC） on pro-inflammatory cytokines released by microglia. Methods Microglia were stimulated with lipopolysaccharide （LPS）, FAC, or LPS ＋ FAC, the concentra tion of interleukin 1β （IL 1β） and tumour necrosis factor-α （TNF-α） in nutrient medium was then detected using enzyme-linked immunoabsorbent assay. Results Almost no releasing of cytokine was found in both FAC group and the control; in LPS group, the dilivery of IL-1β and TNF-α was increased as compared with the control （F= 38. 864,102. 504;q=8. 204,14. 369;P〈0.01）, but much lower than that in LPS＋FAC group （q=4. 517,5. 610;P〈0.01）. Conclusion FAC can enhance activated mieroglia to release a great quantity of pro-inflammatory cytokine.

关 键 词：枸橼酸铁铵 小神经胶质细胞 白细胞介素1Β 肿瘤坏死因子Α 脂多糖类 

分 类 号：R742.5[医药卫生—神经病学与精神病学]