ALOX5AP基因多态性与缺血性脑卒中的相关性  

ASSOCIATION BETWEEN POLYMORPHISM OF ALOX5AP GENE AND ISCHEMIC STROKE

作　　者：孙蕾[1] 李聪[1] 宋博[1] 周畅[1] 王海萍[1] 张晨[1] 

机构地区：[1]青岛大学医学院附属医院神经内科,山东青岛266003

出　　处：《青岛大学医学院学报》2012年第3期207-210,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省自然科学基金资助项目(ZR2010HM061)

摘　　要：目的了解5-脂氧合酶激活蛋白(ALOX5AP)基因的SG13S114和SG13S32两个位点基因多态性与缺血性脑卒中(IS)的相关性。方法采用病例-对照研究及聚合酶链反应限制性片段长度多态性分析(PCR-RFLP)的方法,检测440例IS病人和486例与其年龄、性别相匹配的健康体检者(对照组)SG13S114T/A和SG13S32C/A两个位点基因多态性,比较两组基因型及等位基因频率之间的差异。采用多因素Logistic回归分析方法,分析基因型、等位基因与IS的相关性。结果 IS组病人SG13S114AA基因型及A等位基因频率高于对照组,差异有显著意义(χ2=7.86、15.68,P<0.01,OR=1.48,95%CI=1.22～1.81);SG13S32C/A基因型及等位基因频率差异无显著意义(P>0.05)。结论 ALOX5AP基因SG13S114T/A多态性与IS相关,A等位基因增加IS的发病风险。Objective To understand the correlation between polymorphisms of SG13Sl14 （rs1050739） and SG13S32 （9551963） in two sites of ALOXSAP and ischemic stroke. Methods Using a case-control study and restrictive fragment length polymorphism （PCR-RFLP）, 440 stroke patients and 486 healthy individuals matching the age and sex were enrolled. The poly morphisms of SG13Sl14 T/A and SG13S32 C/A were compared between the two groups in terms of their genotype and allele frequency. The association of genotype and allele with ischemic stroke was analyzed by logistic regression analysis. Results In the ischemic stroke group, the genotype of SG13Sl14 AA and A allele frequency were higher than that in the control （χ2= 7.86, 15.68;P〈0.01 ;OR= 1.48;95%CI= 1.22- 1.81）, but the difference between SG13S32 C/A genetype and allele frequency was not significant. Conclusion The polymorphism of SG13Sl14 in ALOX5AP gene is associated with ischemic stroke, which in creases the risk of incidence of this condition.

关 键 词：脑血管意外 5-脂氧合酶激活蛋白 多态性 单核苷酸 

分 类 号：R743.3[医药卫生—神经病学与精神病学]