改良式Hp感染萎缩性胃炎大鼠模型的建立  

ESTABLISHMENT OF MODIFIED RAT MODEL WITH CHRONIC ATROPHIC GASTRITIS INFECTED BY HELICOBACTER PYLORI

作　　者：唐旭东[1] 张翠萍[1] 张琪[1] 赵坤[1] 孙向红[1] 田字彬[1] 

机构地区：[1]青岛大学医学院附属医院消化内科,山东青岛266003

出　　处：《青岛大学医学院学报》2012年第3期247-249,252,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的建立较理想的幽门螺杆菌(Hp)感染慢性萎缩性胃炎(CAG)大鼠模型。方法雄性Wistar大鼠60只,随机分为Hp感染及水杨酸钠乙醇灌胃组(模型组)、正常对照组(A组)、单纯Hp感染组(B组)、单纯水杨酸钠乙醇灌胃组(C组)。采用灌胃接种Hp菌株(SS1)及20g/L水杨酸钠与体积分数0.60的乙醇混合溶液方法建立Hp感染CAG模型,共14周。检测各组Hp定植情况,并对胃窦黏膜病理组织学各项指标进行评分。结果模型组及B组胃窦黏膜均有Hp定植。模型组胃窦黏膜变薄,固有层腺体中度减少伴有中度慢性活动性炎症。模型组慢性炎症评分、活动性炎症评分及固有腺减少评分均明显高于A、B组(χ2=47.632～48.276,q=6.514～11.191,P<0.01),而与C组比较,差异无显著性(P>0.05)。结论采用Hp感染大鼠及水杨酸钠与乙醇混合溶液灌胃的方法建立Hp感染CAG大鼠模型,其病因、病理变化与人类CAG病变相似,可作为研究Hp感染CAG发病机制及药物干预治疗CAG较合适的动物模型。Objective To create a more ideal rat model of chronic atrophic gastritis （CAG） infected by Helicobacter pylori （Hp）. Methods Sixty male Wistar rats were divided into four groups in random-group D （model group, Hp, sodium salicy late and ethanol group）, group A （normal control group）, group B （merely-Hp infected group）, and group C （sodium salicylate and ethanol group）. The Hp-infected CAG models were created by intragastric vaccination of Hp strain （SS1） and intragastric ad ministration of mixed solution of sodium salicylate and ethanol for 14 weeks. The field planting in each group was detected and the histopathological markers of mucosa of gastric antrum were scored. Results Colonization of Hp in gastric mucosa was found in both model group and group B. The antral gastric mucosa in model group became thinner, the numbers of glands in lamina propria moderately decreased, accompanying with moderately chronic active inflammation. The score of chronic inflammation, active in flammation and decrease of intrinsic gland in model group was significantly higher than that in groups A and B （χ2= 47. 632-- 48. 276;q=6. 514-11. 191 ;P〈0.01）, compared with group C, the difference was not significant （P〉0.05）. Conclusion The causes of disease and pathological changes in rat models of CAG that created by Hp infection combined with mixed solution of sodi- um salicylate and ethanol are similar to that found in human subjects. This can be used as a suitable animal model in researching the mechanism of CAG due to Hp infection and drug therapy for this condition.

关 键 词：螺杆菌感染 胃炎 萎缩性 模型 动物 大鼠 WISTAR 

分 类 号：R-332[医药卫生]