NR2E1促神经母细胞瘤细胞分裂增殖的效应研究  

Effect of NR2E1 on the division and proliferation of neuroblastoma cells

作　　者：朱海霞[1] 马楠[2] 焦春红[2] 薛蔚雯[2] 曾赵军[2] 

机构地区：[1]中南大学湘雅三医院神经内科,长沙410013 [2]中南大学生物科学与技术学院分子生物学研究中心,长沙410078

出　　处：《中南大学学报（医学版）》2012年第6期549-554,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81172154);湖南省自然科学基金(2011JJ5014)~~

摘　　要：目的:探讨核蛋白样转录因子核受体亚家族2组E成员1(nuclear receptor subfamily 2 group E member 1,NR2E1)对儿童神经母细胞瘤细胞株IMR-32生长、分裂、增殖的影响。方法:应用LipofectamineTM2000将构建的针对核蛋白样转录因子NR2E1的shiRNA质粒载体转染神经母细胞瘤细胞株IMR32,并通过细胞计数法观察细胞生长抑制效应,采用细胞免疫荧光染色检测神经母细胞瘤细胞株IMR32细胞分裂蛋白的表达。结果:核蛋白样转录因子NR2E1的shiRNA质粒转染神经母细胞瘤细胞株IMR32 48 h后,该细胞株生长缓慢;相关细胞核分裂蛋白表达受到明显的抑制。结论:核蛋白样转录因子NR2E1的shiRNA干扰质粒转染神经母细胞瘤细胞株IMR32后,抑制了神经母细胞瘤细胞IMR32的分裂和增殖。Objective： To explore the effects of nuclear protein-like transcription factor nuclear receptor subfamily 2 group E member 1 （NR2E1） on the growth, division, and proliferation of neuroblastoma cell line IMR32. Methods： A NR2E1 shiRNA plasmid vector was constructed and transfected into neuroblastoma cell line IMR32 using lipofedamineTM2000. Subsequent cell growth was measured by cell counting and the protein expression of somatic nuclear division was examined by immunofluorescent staining. Results： At 48 h after the neuroblastoma cells IMR32 were transfected with NR2EI-shiRNA vector, the related nuclear division protein and the proliferation of the transfected cells IMR32 were remarkably depressed. Conclusion： Cells division and proliferation of neuroblastoma cell line IMR32 is inhibited through transfection with the NR2E 1-shiRNA plasmid vector.

关 键 词：神经母细胞瘤 核蛋白样转录因子NR2E1 细胞分裂 

分 类 号：R739.4[医药卫生—肿瘤]