奥曲肽联合氟脲嘧啶脱氧核苷或顺铂对SMMC-7721肝癌细胞Fas和FasL蛋白表达的影响  

Influence of Octreotide combined with Fluorodeoxyuridine or Cisplatin on Fas and FasL expression of SMMC-7721 hepatic cancer cells

作　　者：王永峰[1] 张水军[1] 李文涛[1] 

机构地区：[1]郑州大学第一附属医院肝胆外科,郑州市450052

出　　处：《中华实用诊断与治疗杂志》2012年第7期674-676,共3页Journal of Chinese Practical Diagnosis and Therapy

摘　　要：目的观察奥曲肽联合氟脲嘧啶脱氧核苷或顺铂对肝癌细胞株SMMC-7721凋亡的影响,并探讨其治疗机制。方法体外培养SMMC-7721肝癌细胞株,根据干预药物分为6组,分别为阴性对照组(A组)、氟脲嘧啶脱氧核苷组(B组)、顺铂组(C组)、奥曲肽组(D组)、奥曲肽+氟脲嘧啶脱氧核苷组(E组)与奥曲肽+顺铂组(F组),分别干预SMMC-7721细胞24,48,72,96h后,应用酶联免疫检测仪测定各组吸光值并统计细胞抑制率,采用免疫组织化学方法检测各组SMMC-7721细胞表面Fas及FasL蛋白表达变化。结果药物作用24,48,72,96h时,B,C,D,E,F组SMMC-7721肝癌细胞抑制率及Fas和FasL蛋白表达明显高于A组(P<0.05);E组较B组,F组较C组抑制率明显增加,Fas及FasL蛋白表达明显增强(P<0.05)。结论上调肿瘤细胞表面Fas及FasL的表达是奥曲肽协同氟尿嘧啶脱氧核苷或顺铂抑制SMMC-7721细胞增殖,发挥抗肿瘤作用的可能机制之一。Objective To explore the influence of combination of Octreotide, Fluorodeoxyuridine and Cisplatin on the apoptosis of hepatic cancer cell SMMC-7721 in vitro and the therapeutic mechanism. Methods SMMC-7721 cells were cultured in vitro and were divided into six groups according to intervention medicine, group A being intervened by negative culture medium, group B by Fluorodeoxyuridine, group C by Cisplatin, group D by Octreotide, group E by Octreotide and Fluorodeoxyuridine, and group F by Octreotide and Cisplatin. After SMMC-7721 cells were intervened for 24, 48, 72, and 96 hours, the cell inhibition rate was detected with Thermo Labsystems and the changes of Fas and FasL protein expression on the SMMC-7721 cells were detected with immunohistochemieal method. Results The inhibitory rate and the expression of Fas and FasL protein were higher after being intervened for 24, 48, 72 and 96 hours in groups B, C, D and F than those in group A （P〈0.05）, were higher in group E than those in group B, and higher in group F than those in group C （P〈0.05）. Conclusion Octreotide combined with Fluorodeoxyuridine or Cisplatin can obviously inhibit the cell apoptosis rate. The up-regulation of Fas and FasL expression is one of possible mechanisms of hepatic cell apoptosis.

关 键 词：肝癌 奥曲肽 氟脲嘧啶脱氧核苷 顺铂 凋亡 抗癌机制 

分 类 号：R735.7[医药卫生—肿瘤]