阿尔茨海默病患者尿液中相关神经丝蛋白含量的诊断价值  

Value of Alzheimer-associated neuronal thread protein level in urine for diagnosing Alzheimer＇s disease

作　　者：朱瑞[1] 袁欣瑞[1] 彭丹涛[1] 

机构地区：[1]卫生部北京医院神经内科,100730

出　　处：《中华老年医学杂志》2012年第7期575-577,共3页Chinese Journal of Geriatrics

基　　金：国家自然科学基金（30971000）;首都科学发展基金（2007-3011）

摘　　要：目的 探讨阿尔茨海默病（AD）患者尿液中Alzheimer相关的神经丝蛋白（AD7c—NTP）的含量在AD诊断中的价值。方法收集就诊于我院门诊AD患者及健康体检者的尿液标本450例，其中AD组共257例，轻度131例、中重度126例，健康对照组193例，应用双抗体夹心酶联免疫吸附法测定尿样标本中AD7c—NTP水平。结果轻度、中重度AD组尿样标本中AD7c—NTP含量为（1．94±0．74）μg／L和（3．92±0．86）μg／L，健康对照组为（0．65士0．80）／μg／L，轻度和中重度AD组比较，差异均有统计学意义（t=1．727，P〈0．001），三组比较差异有统计学意义（F=13．520，P〈0．001）。轻度AD组尿样标本中AD7c—NTP含量与MMSE呈负相关（r=-0．23，P=0．006），而中重度AD组尿样标本中AD7c—NTP含量与MMSE无明显的相关性（r=0．59，P=0．113）。根据ROC曲线，确定1．50ptg／L为尿AD7c—NTP诊断AD的切点，对应的灵敏度和特异度分别为90．6％和91．8％，ROC曲线下面积为0．94，95％可信区间为0．91～0．97。结论尿AD7c-NTP含量检测可作为AD辅助诊断的方法之一。Objective To investigate the value of Alzheimer-associated neuronal thread protein （AD7c-NTP） level in urine for diagnosing Alzheimer＇s disease （AD）. Methods The urine samples of 450 subjects were collected from out-department of our hospital. There were 257 people with AD diseases （131 mild cases, 126 moderate and severe cases） and 193 healthy control. ELISA was applied to test the level of AD7c-NTP in urine samples. Results The levels of AD7c-NTP were （1.94±0. 74）μg/L,（3. 92± 0.86） μg/L and （0. 65 ± 0.80） μg/L in mild AD, moderate and severe AD, healthy control groups, respectively. There were differences among three groups（F=- 13. 520,P〈 0. 001）,and between mild and moderate and severe AD（t= 1. 727,P〈0. 001）. The level of AD7c- NTP was negatively related with MMSE score in mild AD （r = 0.23, P =0. 006）, while no correlation was found between AD7c-NTP and MMSE in moderate and severe AD （r = 0.59, P 0. 113）. Using receiver operating characteristic curve（ROC curve）, the optimal cutoff point of AD7c- NTP in urine for diagnosis of AD was 1.50μg/L, with 90.6% sensitivity and 91.8% specificity, area under the curve was 0.94（95% CI： 0. 91-0.97）. Conclusions The level of AD7c-NTP in urine may be one of parameters for diagnosing AD.

关 键 词：阿尔茨海默病 神经微丝蛋白质类 

分 类 号：R749.16[医药卫生—神经病学与精神病学]