膜联蛋白A5在人宫颈鳞癌不同分化型的表达及临床意义  

Clinical Significance of ANXA5 Expression in Human Uterine Cervical Carcinoma of Different Pathological Differentiation

作　　者：王立平[1] 李欣[2] 许倩[2] 陈龙[2] 王小杰[2] 

机构地区：[1]承德医学院附属医院,067000 [2]承德医学院基础医学研究所

出　　处：《天津医药》2012年第7期648-650,共3页Tianjin Medical Journal

基　　金：河北省科学技术研究与发展计划项目(项目编号:10276142)

摘　　要：目的:检测膜联蛋白A5(ANXA5)在不同分化的宫颈癌细胞中的表达差异,探讨其作为宫颈鳞癌组织分化标志物的可行性。方法:将收集的宫颈鳞癌组织52例HE染色后分为高分化型12例,中分化型25例,低分化型15例。RT-PCR法和WesternBlot法分别在mRNA转录水平和蛋白水平检测ANXA5在各分化型宫颈鳞癌的表达情况;用免疫组织化学法对其在细胞内的定位以及表达量进行检测。结果:随着癌细胞分化从高到低,ANXA5在mRNA水平的表达逐渐增高,且组间多重比较差异均有统计学意义(均P<0.01)。在蛋白水平,高分化型组表达低于中、低分化型组(均P<0.01),中、低分化组之间差异无统计学意义(P>0.05)。ANXA5主要定位于宫颈癌细胞的细胞质中。结论:ANXA5具有作为临床宫颈癌细胞分化标志物的潜在可能。Objective： To study the possibility of annexin A5 （ANXA5） as a differentiation marker of uterine cervical carcinoma by examining expression of ANXA5 in three kinds of pathology differentiation. Methods： Fifty-two specimens were classified as well-differentiated （n=12）, intermediate-differentiated （n=25） and poor-differentiated groups （n=15） according to HE staining. RT-PCR and Western blot methods were used to determine the ANXA5 mRNA and protein expression. The immunohistochemistry was employed to determine the ANXA5 expression. ResUlts： On mRNA level, with the carcinoma cells differentiated from well to poor, ANXA5 mRNA expression increased, and the expression was significantly different between any two groups （P 〈 0.05）. On protein level, ANXA5 expression increased in three groups. The expression of ANXA5 was lower in well-differentiated group than that of moderately- and poor-differentiated groups（P 〈 0.01）, but no sig- nificant difference between moderately-differentiated group and poor-differentiated group （P 〉 0.05）. ANXA5 mainly local- ized in the cytoplasm of cervical cancer cells. Conclusion： ANXA5 maybe a potential marker for differentiation types of uterine cervical carcinoma.

关 键 词：膜联蛋白A5 细胞分化 癌 鳞状细胞 宫颈肿瘤 基因表达 免疫组织化学 逆转录聚合酶链反应 WESTERNBLOT 

分 类 号：R735.34[医药卫生—肿瘤]