脉冲电磁场对大鼠成骨细胞作用的研究  

Effects of Pulsed Electromagnetic Fields on Rat Osteoblasts

作　　者：黄晶晶[1] 杨福军[1] 赵吉 孙元明[1] 刘晓秋[1] 赵阿津[1] 

机构地区：[1]北京协和医学院、中国医学科学院放射医学研究所,天津300192 [2]常州方圆制药有限公司

出　　处：《天津医药》2012年第7期685-687,I0001,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30970867);天津市应用基础及前沿技术研究计划(项目编号:08JCYBJC08500);北京协和医学院研究生创新基金项目(项目编号:院1123);中国医学科学院放射医学研究所所基金(项目编号:ST1108)

摘　　要：目的:研究脉冲电磁场(PEMFs)对大鼠成骨细胞的作用。方法:取新生SD大鼠头盖骨,通过骨组织块培养法分离、培养成骨细胞。取传代第3代细胞分为5组,1-70组、2-70组、3-70组、4-70组、对照组,其中前4组为作用组,接受频率为70Hz,峰值磁场分别为1、2、3、4mT的PEMFs作用,对照组不接受PEMFs作用。按照分组剂量进行离体PEMFs作用(0.5h/d,连续作用4d),作用结束后,对各组分别进行细胞碱性磷酸酶(ALP)活性,核结合因子α1(cbfα1)表达,体外成骨能力指标—骨结节形成能力检测。结果:经PEMFs作用后,与对照组相比,成骨细胞ALP活性降低,cbfα1表达增多,骨结节数量显著增多。结论:PEMFs作用成骨细胞可促进成骨细胞功能分化,增强成骨细胞的细胞外基质矿化。Objective： To study the effect of pulsed electromagnetic fields （PEMFs） on rat osteoblasts. Methods： Osteoblasts were separated from newly born SD rat calvaria and cultured in vitro. The third-generation cells were divided into five groups including 1-70 group, 2-70 group, 3-70 group, 4-70 group and control group. According to the given dose of each group, treated groups were exposed to PEMFs （1, 2, 3 and 4 roT）, and control group was given nothing. After the treatments （0.5 h/d for 4 d）, the alkaline phosphatase （ALP） level, core-binding factor a1 （cbfa1/RUNX2） staining and mineralized nodules staining were detected respectively in five groups. Results： After exposure to PEMFs, ALP activity was significantly reduced in osteoblasts than that of control group. The cbfa1-positive osteoblasts were increased. And the number of mineralized nodules was significantly increased. Conclusion： PEMFs promote osteoblast differentiation to extracellular matrix maturation. PEMFs are contributed to osteoblast extracellular matrix mineralization.

关 键 词：成骨细胞 电磁场 碱性磷酸酶 大鼠 Sprague—Dawley 脉冲电磁场 核结合因子α1 骨结节 

分 类 号：R782.2[医药卫生—口腔医学]