奥美拉唑对肝癌HepG2细胞增殖与凋亡的影响  

Effects of Omeprazole on the Proliferation and Apoptosis of Hepatoma Cell Line HepG2

作　　者：魏艳[1] 梁宁林[1] 朱永军[1] 吴文超[2,3] 刘小菁[2,3] 杨丽[1] 

机构地区：[1]四川大学华西医院消化内科,成都610041 [2]四川大学华西医院心血管疾病研究室,成都610041 [3]四川大学华西医院再生医学研究中心,成都610041

出　　处：《四川大学学报（医学版）》2012年第4期513-516,共4页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅项目(No.2009FZ0097)资助

摘　　要：目的探讨质子泵抑制剂奥美拉唑(omeprazole,OME)对肝癌HepG2细胞增殖和细胞凋亡的影响。方法不同浓度(0、10、20、40、80、160mg/L)的OME作用于HepG2细胞后,分别于不同时间(24h、48h),采用甲基四唑蓝(MTT)法测定OME对HepG2细胞增殖的影响;5-乙炔基-2’-脱氧尿嘧啶核苷(Edu)荧光检测法测定DNA合成期(S期)细胞所占比例;Hoechst33342染色法检测细胞凋亡。结果 MTT结果示,10、20mg/L OME对HepG2细胞增殖无明显抑制,而40、80、160mg/L OME可产生明显抑制作用,其中80mg/L OME作用最强;且相同浓度OME作用下,48h较24h对HepG2的抑制率增加。Edu荧光检测法表明,不同浓度OME处理细胞24h,Edu孵育2h后处于DNA合成期(S期)HepG2细胞比例与对照组比较,在20、40、80、160mg/L组减少(P<0.05)。Hoechst33342染色法表明,与对照组相比,40、80、160mg/L OME处理HepG2细胞24h后,细胞凋亡增加(P<0.05)。结论 OME能抑制肝癌HepG2细胞的增殖,并可促进细胞凋亡。Objective To investigate the effects of omeprazole（OME）,a proton pump inhibitor,on the proliferation and apoptosis of human hepatoma cell line HepG2.Methods HepG2 cells were cultured to the logarithmic phase,and then treated with OME of different concentrations（10,20,40,80,160 mg/L） for 24 h or 48 h.Cell proliferation was evaluated by MTT assay,DNA synthesis was measured with 5-ethynyl-2＇-deoxyuridine（Edu） fluorescent assay and the apoptosis of cells was measured by the Hoechst33342 assay.Results MTT assay showed that OME（40,80 and 160 mg/L concentrations） could inhibit the proliferation of HepG2 cells for 24 h or 48 h treatment（P〈0.05） and 80 mg/L group has strongest effect.Compared with that of 24 h treatment,the same concentration of OME could inhibit HepG2 more significantly with 48 h treatment.After different concentrations of OME treatment for 24 h and then incubation with Edu for 2 h,compared with the control group,the proportion of Cells in S phase in 20,40,80,160 mg/L groups decreased.Hoechst33342 staining demonstrated that treatment with OME（40,80,160 mg/L） for 24 h could significantly promote the cell apoptosis.Conclusion Omeprazole could inhibit human hepatoma cell line HepG2 cell proliferation and promote apoptosis.

关 键 词：奥美拉唑 肝癌 增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]