非酒精性脂肪性肝病患者肠道闭锁小带蛋白1表达水平的测定  

Expression of Zonula occludens-1 protein in intestine in patients with nonalcoholic fatty liver disease

作　　者：戴鑫[1] 吕宗舜[1] 王邦茂[1] 杨雪敏[1] 

机构地区：[1]天津医科大学总医院消化内科,天津300052

出　　处：《临床荟萃》2012年第14期1218-1220,F0002,共4页Clinical Focus

摘　　要：目的检测非酒精性脂肪性肝病(NAFLD)患者中肠道黏膜上皮细胞闭锁小带蛋白1(ZO-1)的表达情况,并探讨其与NAFLD发病的关系。方法选取NAFLD伴转氨酶升高患者14例,NAFLD伴转氨酶正常者24例,健康对照组31例。进行相关生化指标的检测,免疫组化技术检测其肠道黏膜上皮细胞ZO-1蛋白表达情况并分析其与转氨酶水平的相关性。结果 NAFLD转氨酶升高组,NAFLD伴转氨酶正常组及健康对照组体质量指数(BMI),总胆固醇(TC),甘油三酯(TG),空腹血糖(FPG),丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST)3组间差异均有统计学意义。NAFLD伴转氨酶升高组的BMI、TC、TG,FPG,AST,ALT均高于NAFLD伴肝功能正常组;而后者的指标又高于健康对照组。3组在ZO-1蛋白的强阳性表达水平上有统计学意义(χ2=11.731,P<0.01)。健康对照组,NAFLD伴转氨酶正常组,NAFLD伴氨酶升高组,ZO-1蛋白的表达情况依次减低(rs=0.424,P<0.01)。在NAFLD患者中,ZO-1的表达程度与肝功能的升高水平呈负相关(rs=-0.347,P<0.05)。结论肠道黏膜上皮细胞ZO-1蛋白的表达与NAFLD的发生和发展状况密切相关,为"肠-肝轴"机制解释NAFLD的发生发展提供新的证据。Objective To investigate the expression of Zonula occludens I（ZO-1） protein in intestinal epithelial cells in patients with nonalcoholic fatty liver disease（NAFLD） and explore its relationship with the pathogenesis of NAFLD. Methods We examined 14 patients with NAFLD and elevated transaminase, 24 patients with NAFLD but normal transaminase and 31 healthy volunteers. We measured their biochemical characters, assessed the expression of ZO-1 protein in intestinal epithelial cells by immunohistochemical analysis and discussed their relationships with transaminase levels. Results Significant differences in the levels of BMI, TC, TG, FPG, ALT, AST could be found among three groups. The levels of BMI, TC, TG, FPG, AST, ALT in the patients with NAFLD and elevated transaminase were significantly higher than the patients with NAFLD but normal transaminase and the latter ones were higher than the volunteers. The expression of ZO-1 protein was significantly different among the three groups（X^2 = 11. 731, P 〈0.05）. From healthy volunteers,the patients with NAFLD but normal transaminase to the patients with NAFLD and elevated transaminase, the expression of ZO-1 protein were decreasing gradually （ rs = 0. 424, P 〈0.01）. In the patients with NAFLD, the levels of the expression of ZO 1 protein and the increased transaminase level were negatively correlated （ rs = -0. 347, P 〈0.05）. Conclusion The expression of ZO-1 protein in intestinal epithelial cells and the occurrence and development of NAFLD are closely reiated, which can provide new evidence for the hypothesis of the contribution of gut liver axis to the pathogenesis of NAFLD.

关 键 词：脂肪肝 紧密连接部 转氨酶类 

分 类 号：R575[医药卫生—消化系统]