上皮性卵巢癌患者肿瘤组织及血清中YKL-40的表达及临床意义  

The expression and significance of YKL-40 in serum and tumor tissue of patients with epithelial ovarian tumor

作　　者：吴文英[1] 杨艳华[2] 徐春琳[2] 

机构地区：[1]河北省唐山市丰润区人民医院妇产科,唐山064000 [2]河北医科大学第二医院妇产科,石家庄050000

出　　处：《中国优生与遗传杂志》2012年第7期25-26,40,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨甲壳质酶蛋白40(YKL-40)在上皮性卵巢肿瘤发生发展中的作用。方法 (1)免疫组化法检测20例上皮性良性卵巢肿瘤、10例交界性卵巢肿瘤及30例卵巢癌组织中YKL-40的表达情况。(2)酶联免疫吸附法检测三组患者血清中YKL-40的水平。结果 (1)YKL-40在卵巢癌组织的阳性表达率高于良性组(P<0.05);交界组与卵巢癌组无差异(P>0.05),但卵巢癌组YKL-40染色强度高于交界组(P<0.05);早期卵巢癌(Ⅰ、Ⅱ期)组织中阳性表达率低于晚期卵巢癌(Ⅲ、Ⅳ期)组织中阳性表达率(P<0.05),YKL-40组织中表达水平与卵巢癌临床分期呈正相关(P<0.05)。(2)上皮性良性卵巢肿瘤、交界性卵巢肿瘤及卵巢癌患者中,血清YKL-40的中位数分别为41.42、44.34、130.25(μg/L)。卵巢癌组血清YKL-40水平高于前二组,差异有统计学意义(P<0.05),前二组比较差异无统计学意义。卵巢癌患者血清YKL-40浓度与临床分期及CA125浓度呈正相关(P<0.05)。结论 YKL-40蛋白可能参与了卵巢肿瘤的发生发展过程。Objective： To explore the pole of YKL- 40 protein in the epithelial ovarian tumorous genesis and progression. Methods ： （ 1 ） Immunohistochemically was applied to detect YKL - 40 protein in tissues of benign ovarian tumor, borderline ovarian tumor and ovarian carcinoma. （2） The Enzyme -linked Immunosorbent Assay （ELISA） technique was applied to detect serum YKL -40 levels in patients of benign ovarian tumor, borderline ovarian tumor, ovarian carcinoma. Results： （1） The positive expression rate in ovarian carcinoma group was significantly higher than benign ovarian tumor group （ P 〈 0. 05 ）. The positive expression rate of the borderline ovarian tumor and ovarian carcinoma was no statistically difference, but the YKL - 40 expression （ staining percentage scale） was higher in the ovarian carcinoma than in the borderline tumor （ P 〈 0. 05 ）. In ovarian carcinoma patients, the positive expression rate at early stages （ stage I ,Ⅱ） was significantly lower than the advanced stages （ stage Ⅲ, Ⅳ） （P 〈 0.05）. YKL - 40 expression positively correlated with clinical stage in tissue of ovarian carcinoma. （2） In benign ovarian tumor, borderline ovarian tumor and ovarian carcinoma, the serum YKL -40 median levels were 41.42, 44. 34, 130.25 （μg/L） respectively. The median YKL -40 levels of ovarian carcinoma group was significantly higher than each of the former two groups （ P 〈 0. 05）. The former two groups were further compared, no statistically significant difference respectively （P 〉 0. 05）. In ovarian carcinoma group, the YKL - 40 levels was positively correlated with the concentration of CA125. Conclusion： The YKL -40 protein may participate the epithelial ovarian tumorous genesis and progression.

关 键 词：甲壳质酶蛋白40(YKL-40) 上皮性卵巢肿瘤 免疫组织化学法 酶联免疫吸附试验 

分 类 号：R737.31[医药卫生—肿瘤]