Simultaneous determination of seven alkaloids and two flavonoid glycosides in Wuzhuyu decoction by RP-HPLC-DAD  

RP-HPLC-DAD法同时测定吴茱萸汤中7个生物碱和2个黄酮苷含量(英文)

作　　者：胡传芹[1] 杨秀伟[1] 

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室,药学院天然药物学系,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》2012年第4期338-344,共7页中国药学（英文版）

基　　金：National Sciences and Technology Program of China (Grant No. 2011BAI07B08,2006BAI06A01-02,2006BAI08B03-09)

摘　　要：A reversed-phase high-performance liquid chromatography （RP-HPLC） method with diode array detector was established to simultaneously determine nine bioactive compounds in Wuzhuyu decoction, namely dehydroevodiamine, isorhanmetin-7-O- rutinoside, 10-hydroxyrutaecarpine, diosmetin-7-O-13-D-glucopyranoside, evodiamine, rutaecarpine, 1-metbyl-2-n-nonyl-4（1H） quinolone, evocarpine, and dihydroevocarpine. The RP-HPLC assay was performed on a reversed-phase Cl8 column with a gradient elution. The mobile phases consisted of methanol and water containing 1% （v/v） acetic acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 300 nm. All calibration curves showed good linearity （r2〉0.9941） within test ranges. The results of intra-day precision, inter-day precision, and accuracy were satisfactory. The overall recoveries were in the range of 90.13%-102.48%. The method was successfully applied to quantify nine bioactive compounds in six samples. Baseline resolution for all nine major compounds was achieved. This method could be used as a suitable quality control assay for Wuzhuyu decoction.建立反相高效液相色谱-二极管阵列检测 (RP-HPLC-DAD) 法同时测定经典名方吴茱萸汤中7个生物碱 (去氢吴茱萸碱、10-羟基吴茱萸次碱、吴茱萸碱、吴茱萸次碱、1-甲基-2-正壬基-4(1H)喹诺酮、吴茱萸卡品碱和二氢吴茱萸卡品碱) 和2个黄酮苷 (异鼠李素-7-O-芸香糖苷和香叶木素-7-O-β-D-吡喃葡萄糖苷)的含量。RP-HPLC分离应用反相C18 色谱柱, 甲醇-1%醋酸水溶液梯度洗脱, 流速为1.0 mL/min, 检测波长为300 nm。上述9个分析物达到基线分离, 标准曲线线性关系良好(r2>0.9941), 日内和日间精密度和准确度符合分析方法学要求, 加样回收率为90.13%-102.48% (RSDs<3.6%)。经方法学验证, 所建立的方法适用于吴茱萸汤物质基础的质量控制。

关 键 词：High-performance liquid chromatography Wuzhuyu decoction ALKALOIDS Flavonoid glycosides 

分 类 号：R284.1[医药卫生—中药学]