p73基因不同启动子区的结合元件和甲基化谱的差异比较  

Different transcription factors profile and methylation status of p73 promoter region

作　　者：唐林[1,2] 聂伟伟[1,2] 韦凤[1,2] 王丽[1,2] 陈龙邦[1,2] 管晓翔[1,2] 

机构地区：[1]南京大学医学院临床学院 [2]南京军区南京总医院肿瘤内科,南京210002

出　　处：《癌症进展》2012年第3期280-284,共5页Oncology Progress

基　　金：国家自然科学基金(项目编号:81141094;30870962);江苏省自然科学基金(项目编号:BK2011656)

摘　　要：目的 p73基因含有两种不同的启动子结构,转录生成一些具有不同甚至相反的功能特性的异构体。利用生物信息学相关软件比较这两种p73基因启动子区的CpG岛以及潜在的转录因子结合位点的差异。方法利用GenBank获取人p73启动子序列,在线MethPrimer软件分析p73启动子序列中甲基化CpG岛;启动子在线分析软件Promoter2.0,Neural Network Promoter Prediction,Promoter SCAN,TFSEACH分析p73基因启动子区序列中潜在的转录因子结合位点。结果 p73基因有两个启动子结构。promoter1序列中含5个CpG岛,promoter2序列中含1个CpG岛。启动子相关软件分析评分在95分以上时,promoter1序列有11个潜在的转录因子结合位点。promoter2序列有9个潜在的转录因子结合位点。结论 p73基因启动子的甲基化区域以及潜在的转录因子结合位点的差异可能是导致转录产物的功能差异的重要因素。Objective p73 gene contains two different promoter regions , which transcribe a number of isomers of different or even opposite features. Bioinformatics tools were used to predict CpG islands and the transcription factor binding sites in the promoter regions of p73 gene. Methods The promoter sequence of p73 was obtained from the Genbank, and then the CpG islands and transcription factor binding sites were predicted by MethPrimer, Neural Net- work Promoter Prediction, Promoter SCAN, Promoter 2.0 and TFSEACH. Results The p73 gene possesses two promoters： pl and p2.5 CpG islands and 11 transcription factor binding sites with scores higher than 95 were predic- ted in the promoterl of human p73. And on-line analyzing software forecast that there was 1 CpG island and 9 tran- scription factor binding sites with scores higher than 95 in the promoter2 of human p73. Conclusion Differences of CpG islands and the transcription factor binding sites in the promoter regions of p73 may be the key to functional diver- sity in transcription products.

关 键 词：p73启动子区 DNA甲基化 转录因子结合位点 生物信息学分析 

分 类 号：R730[医药卫生—肿瘤]