脂多糖联合α-干扰素抑制体外白血病细胞株U937的增殖及凋亡效应研究  

LPS combined IFN-α inhibition of proliferation and apoptosis effects of U937 cell in vitro leukemia

作　　者：刘倩平[1] 邹三鹏[1] 章志福[1] 

机构地区：[1]南方医科大学附属花都医院肿瘤科,510800

出　　处：《中国实用医药》2012年第16期58-59,共2页China Practical Medicine

摘　　要：目的实验观察α-干扰素(IFN-α)和/或脂多糖(LPS)对体外白血病细胞株U937的增殖及凋亡效应。方法 MTT法检测IFN-α和/或LPS对U937细胞增殖效应的影响;同时采用流式细胞仪对U937细胞的凋亡率的影响。结果等剂量合用IFN-α和LPS后对U937细胞的增殖率和凋亡率均有明显的升高作用(P<0.05),具有统计学意义。结论 IFN-α和LPS联用后对白血病细胞株U937的增殖抑制率及凋亡率有明显增强作用。Objective Experimental observation of IFN-α and/or LPS inhibition of proliferation and apoptosis effects of U937 cell in vitro leukemia. Methods MTY assay of IFN-alpha and/or LPS on U937 cell proliferation effect; using flow cytometry to detect the apoptosis rate of U937 cells. Results The same dose combination of IFN-ct and LPS significantly increased the proliferation rate and apoptosis rate of U937 cells （ P 〈 0.05 ）. Conclusion IFN-cxassoeiated with LPS significantly enhanced the effect of proliferation inhibition rate and apoptosis rate of U937 in leukemia cell line.

关 键 词：脂多糖 Α-干扰素 U937细胞 增殖 凋亡 

分 类 号：R733.7[医药卫生—肿瘤]