丙肝病毒基因在肝门部胆管癌组织中的表达及其意义  

The expression of hepatitis C virus gene in hilar cholangiocarcinoma and implication

作　　者：陈汝福[1] 邹声泉[1] 赵西平[2] 

机构地区：[1]同济医科大学附属同济医院普外科,武汉430030 [2]同济医科大学附属同济医院临床免疫室,武汉430030

出　　处：《中华实验外科杂志》2000年第3期223-224,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　探讨丙肝病毒核糖核酸 (HCVRNA)及蛋白在肝门部胆管癌组织中的表达及其生物学意义。方法　应用套式逆转录 多聚酶链反应 (nestedRT PCR)和免疫组织化学技术检测 36例肝门部胆管癌患者癌组织和癌旁组织中HCVRNA、非结构蛋白 (NS5)蛋白水平的表达。结果　 36例肝门部胆管癌患者癌组织和癌旁组织中HCVRNA和NS5蛋白阳性表达率为 83.3% ( 30 /36)、47.2 % ( 1 7/36) ,2种组织间的差异有非常显著性 (P <0 .0 1 )。癌组织和癌旁组织中NS5蛋白阳性表达率分别为 36.1 % ( 1 3/36)、2 2 .2 % ( 8/36) ,2种组织间差异无显著性 (P >0 .0 5)。HCVRNA阳性表达与肝门部胆管癌的发生部位、病理类型、分化程度、有无转移、丙氨酸转移酶 (ALT)高低以及有无输血史、乙型肝炎表面抗原阳性 [HBsAg( + ) ]、肝硬化、黄疸等无关 (P >0 .0 5) ,仅与是否伴有血清抗HCV阳性有关 (P <0 .0 5)。结论　肝门部胆管癌组织中有HCV基因及其产物的高表达 。Objective\ To elucidate the expression of hepatitis C virus HCV RNA and NS5 protein in hilar cholangiocarcinoma (HCC) and its biological value. Methods\ Nested reverse transcriptase polymerase chain reaction (nested RT PCR) and immunohistochemical assays were used to detect the expression of HCV RNA, NS5 protein in cancerous and noncancerous tissues of 36 cases of HCC. Results\ The positive expression rate of HCV RNA in cancerous and noncancerous was 83.3% and 47.2% respectively ( P <0.01), but the positive expression rate of NS5 in cancerous and noncancerous tissues was 36.1% and 22.2% respectively ( P >0.05). HCV RNA expression was correlated with anti HCV positive ( P < 0.05 ), but had no relation to clinical pathological types, extent of differentiation, existence of metastasis, blood transfusion, HBsAg positive, liver cirrhosis, jaundice, level of ALT ( P >0.05). Conclusion\ HCV gene and its product were highly expressed in HCC. HCV infection was associated with the pathogenesis of HCC.\;

关 键 词：肝门部胆管癌 丙型肝炎病毒 RNA NSS蛋白 

分 类 号：R735.8[医药卫生—肿瘤]