腹膜乳斑在胃癌腹膜播散过程中的作用  

The role of peritoneal milky spots in gastric carcinoma peritoneal dissemination

作　　者：安伟德[1] 胡祥[1] 郭文斌[2] 沈忠义[1] 

机构地区：[1]大连医科大学附属第一医院普外科,116011 [2]大连市中心医院

出　　处：《中华实验外科杂志》2000年第3期243-244,共2页Chinese Journal of Experimental Surgery

基　　金：辽宁省自然科学基金资助项目! ( 981 0 50 0 1 0 6)

摘　　要：目的　研究胃癌腹膜播散过程中腹膜乳斑的作用。方法　在 3组实验动物中 ,分别向大鼠腹腔内接种经 5 溴脱氧尿苷 ( 5 bromodeoxyurdine ,5 BrdU)标记的Sy86B裸鼠可移植性人胃癌细胞 ,每毫升数量分别为 1× 1 0 5 个、1× 1 0 6 个和 1× 1 0 7个。应用免疫组织化学染色 ,观察癌细胞的分布。计数网膜乳斑或非乳斑区标记细胞的数量 ,确定癌细胞的分布。结果　在乳斑或非乳斑区平均标记细胞数 3组的比率分别是 1 63∶1 ( 1× 1 0 5 个 /ml组 )、2 80∶1 ( 1× 1 0 6 个 /ml组 )和 697∶1 ( 1× 1 0 7个/ml组 )。在乳斑或非乳斑区标记细胞数比较差异有非常显著性 (P <0 .0 1 )。结论　标记细胞选择性地侵入网膜乳斑。在胃癌腹膜播散的早期 。Objective\ To investigate the role of peritoneal milky spots in gastric carcinoma peritoneal dissemination. Methods\ Thirty cyclosporin A treated SD rats were inoculated introperiiotonealy with human gastric cancer sy86B cells, labeled with bromodeoxyuridine (BrdU), of 1×10 5 ( n =10), 1×10 6 ( n =10) and 1×10 7 ( n =10) respectively. Immunohistochemically the distribution of the labeled cells in the greater omentum taken from the rats was observed with an anti BrdU antibody. The number of the BrdU labeled cells at each milky spot and non milky spot site in the omentum was quantified to determine the distribution of the cancer cells. Results\ The ratio of the mean labeled cell number in the millky spot sites to the non milky spot sites were 163∶1 (1×10 5), 80∶1(1×10 6) and 697∶1 (1×10 7) respectively. There were statistically significant differences in the number of BrdU labeled cells/mm 2 between milky spot sites and non milky spot sites. Conclusion\ Cancer cells spread in the peritoneal cavity and infiltrate selectively milky spots in the early stage of gastric carcinoma peritoneal dissemination.\;

关 键 词：腹膜播散 胃癌 腹膜乳斑 5-BrdU 

分 类 号：R735.2[医药卫生—肿瘤]