人膀胱癌细胞波状层次生长的研究  

Study of wavy laminar growth of humn urinary bladder cancer cell colony in vitro

作　　者：邓国宏[1] 丛延广[1] 刘俊康[1] 徐启旺[1] 袁泽涛[1] 

机构地区：[1]第三军医大学生物波研究中心,重庆400038

出　　处：《第三军医大学学报》2000年第5期465-469,共5页Journal of Third Military Medical University

摘　　要：目的　观察人膀胱癌细胞 (BIU)在体外培养条件下的生长有序行为。方法　将BIU细胞悬液进行局限区域接种 ,待其形成贴壁生长的群落后进行宏观和显微动态观察及参数测量 (扩展直径、扩展速率、细胞形态、细胞平均密度、细胞平均大小、脱氢酶活性、对pH的敏感性等 )。结果　体外细胞培养条件下 ,局限区域生长的BIU细胞群落能出现明显的层次特性 ,不同区域细胞的形态、大小、密度、脱氢酶活性以及对pH的敏感性均存在差异。结论　空间封闭和生物耗散导致细胞自组织 ,其结果表现为生长有序行为。Objective To observe the ordered growth behavior of human urinary bladder cancer cell line under culture in vitro . Methods The suspension of human urinary bladder cancer cells was inoculated to the localized areas. After the cells grew alone the wall to form colonies, macroscopic and microscopic observations of the spreading diameter, spreading rate, cell shape, average cell density, average cell size and dehydrogenase activity and its sensitivity to pH were dynamically performed. Results Under culture conditions, obvious laminar structure appeared in the locally growing colonies of human urinary bladder cancer cells and there was difference of the shape, size, density and dehydrogenase activity and its sensitivity to pH among the cells in different zones. Conclusion Space closing and biodissipation lead to the self organization of the human urinary bladder cancer cells. Consequently they show ordered growth behavior. In this paper, a simple and controlled model of wavy growth of human cells is provided.

关 键 词：膀胱癌 癌细胞培养 生物波 肿瘤细胞生长 

分 类 号：R737.14[医药卫生—肿瘤]