Biological pretreatment of corn stover by solid state fermentation of Phanerochaete chrysosporium  

作　　者：Jian ZHANG Xin REN Wenqun CHEN Jie BAO 

机构地区：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China

出　　处：《Frontiers of Chemical Science and Engineering》2012年第2期146-151,共6页化学科学与工程前沿（英文版）

基　　金：Acknowledgements This research was supported by the State Key Development Program for Basic Research of China （Grant No. 2011CB707406）, the National Natural Science Foundation of China （Grant No. 20976051）, Ministry of Education of China （Grant No. 20090074110013）, the Shanghai Leading Academic Discipline Project （B505）, and the Fundamental Research Funds for the Central Universities of China （WF0913005）.

摘　　要：Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the super- molecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus Phanerochaete chrysosporium was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes （LiP and MnP） were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are usefuI to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the super- molecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus Phanerochaete chrysosporium was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes （LiP and MnP） were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are usefuI to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.

关 键 词：biological pretreatment Phanerochaete chry- sosporium solid state fermentation biorecalcitrance hydrolysis yield 

分 类 号：X172[环境科学与工程—环境科学] S823.5[农业科学—畜牧学]