Cathepsin B抑制剂对反复惊厥新生大鼠海马组织Clusterin表达的干预作用  

Intervention Effect of Cathepsin B Inhibitor on Expression of Clusterin in Neonatal Rats Hippocampus after Recurrent Neonatal Seizures

作　　者：陈情情[1] 倪宏[1] 

机构地区：[1]苏州大学附属儿童医院儿科医学研究所小儿神经实验室,江苏苏州215003

出　　处：《实用儿科临床杂志》2012年第12期907-909,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(30870808);江苏省自然科学基金(BK2007509;BK2010233);江苏省卫生厅医学重点人才项目(RC2011113)

摘　　要：目的探讨新生期大鼠反复惊厥后海马丛生蛋白(Clusterin)的表达及溶酶体蛋白酶抑制剂Cathepsin B(CBI)对其表达的干预作用。方法 6日龄SD大鼠随机分为3组:对照组、惊厥组(RS组)、CBI组,每组6只。RS组新生大鼠每日吸入0.04～0.05 mL三氟乙醚诱导其惊厥发作5次,每次间隔30 min,连续9 d;对照组同样操作但不吸入三氟乙醚;CBI组惊厥前腹腔注射CBI(2μL,0.5 g.L-1),采用同样方法吸入三氟乙醚诱导其惊厥发作。于出生35 d(P35)处死3组大鼠,并取其海马组织。采用免疫印迹技术检测其Clusterin的表达。3组蛋白水平比较采用SPSS 17.0软件进行方差分析。结果 RS组海马中Clusterin表达(1.19±0.10)明显高于对照组(0.64±0.24),两两比较差异有统计学意义(P<0.01)。CBI组海马Clusterin表达(0.80±0.16)明显低于RS组,差异有统计学意义(P<0.01),CBI组海马Clusterin表达与对照组比较差异无统计学意义。结论惊厥后Clusterin蛋白表达水平明显上调,表明Clusterin参与了新生大鼠反复惊厥后所致脑损伤的分子机制,CBI对其表达有明显干预作用。Objective To explore the expression of Clusterin（CLU） in neonatal rats hippocampus after recurrent neonatal seizures and the intervention efficacy of Cathepsin B inhibitor（CBI）. Methods Six-day-old Sprague-Dawley（SD） rats were randomly divided into 3 groups,including recurrent seizure group（RS group,n=6）,CBI-treated seizure group（CBI group,n=6）,and control group（n=6）.Rats in RS group were subjected to 5 attacks of seizures induced by using flurothyl for 9 d,at intervals of 30 min,while rats in control group did without flurothyl.In CBI group,CBI（2 μL,0.5 g·L-1）was administered every day before seizures were induced.Western blot was employed to determine Clusterin expression at P35.SPSS 17.0 software was used to analyze the data. Results There was higher expressions of Clusterin in RS group（1.19±0.10） than that in control group（0.64±0.24）（P〈0.01）.There was lower expression of Clusterin in CBI group（0.80±0.16） than that in RS group（P〈0.01）.There was no significant difference between CBI group and control group. Conclusions Increased Clusterin expression may be involved in molecular mechanism induced by brain injury after recurrent seizures.CBI has significant intervention efficacy on the expression of Clusterin.

关 键 词：CLUSTERIN 惊厥 组织蛋白酶B抑制剂 大鼠 新生 

分 类 号：R742.1[医药卫生—神经病学与精神病学]